Aloe L, Alleva E, De Simone R
Institute of Neurobiology, CNR, Rome, Italy.
Behav Brain Res. 1990 Jun 18;39(1):53-61. doi: 10.1016/0166-4328(90)90120-4.
Nerve growth factor (NGF) immunoreactivity was detected in the hypothalamus of adult male mice. NGF-immunoreactive cell bodies were examined through consecutive brain sections, and it was found that most of the NGF-positive cells were located in the dorsomedial and mediolateral portions of the hypothalamus. Tissue culture bioassays showed that hypothalamic extract elicits neurite outgrowth from both chick sensory ganglia and rat superior cervical ganglia neurons, and that these effects are inhibited by addition of NGF antibodies. Our results also showed that intermale aggressive behaviour induced by 6-8 weeks of social isolation induces an NGF increase in the hypothalamic area, which is not abolished by sialoadenectomy, suggesting that the increased brain NGF is locally synthesized, and does not come from salivary sources. Likewise, the level of NGF in the hypothalamus of adrenalectomized fighting mice increased, although to a much lesser extent, when compared to hypothalamic levels of sham-operated fighting mice. The present results and a recent report showing that aggressive behaviour causes an increase of mRNANGF in hypothalamic areas are discussed in relation to a possible functional role of NGF in these brain structures.
在成年雄性小鼠的下丘脑检测到神经生长因子（NGF）免疫反应性。通过连续的脑切片检查NGF免疫反应性细胞体，发现大多数NGF阳性细胞位于下丘脑的背内侧和中外侧部分。组织培养生物测定表明，下丘脑提取物可诱导鸡感觉神经节和大鼠颈上神经节神经元的神经突生长，并且添加NGF抗体可抑制这些作用。我们的结果还表明，6-8周的社会隔离诱导的雄性间攻击行为会导致下丘脑区域的NGF增加，而唾液腺切除术后这种增加并未消除，这表明脑内增加的NGF是局部合成的，并非来自唾液。同样，与假手术的打斗小鼠的下丘脑水平相比，去肾上腺的打斗小鼠下丘脑的NGF水平有所增加，尽管程度要小得多。结合NGF在这些脑结构中可能的功能作用，讨论了本研究结果以及最近一份表明攻击行为会导致下丘脑区域NGF mRNA增加的报告。
