开发核 SSR 标记用于孤岛灌木臭牡丹（马鞭草科）和广泛分布的臭牡丹。

Development of nuclear SSRs for the insular shrub Clerodendrum izuinsulare (Verbenaceae) and the widespread C. trichotomum.

机构信息

Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.

出版信息

Am J Bot. 2011 Nov;98(11):e333-6. doi: 10.3732/ajb.1100155. Epub 2011 Oct 24.

DOI:10.3732/ajb.1100155

PMID:22025293

Abstract

PREMISE OF THE STUDY

Nuclear microsatellite primers were developed in the insular shrub Clerodendrum izuinsulare and the closely related widespread C. trichotomum to provide molecular tools to undertake a comparative study of the reproductive systems and genetic structures of the two Clerodendrum species.

METHODS AND RESULTS

Using an improved protocol for isolating codominant compound microsatellite markers, 19 primer sets were developed in C. izuinsulare and C. trichotomum. The primers amplified dinucleotide repeats with one to nine alleles per locus in C. izuinsulare and one to 15 alleles per locus in C. trichotomum. Tests of cross-amplification showed that four to 16 loci could be amplified using these markers in five other species of Clerodendrum that were studied.

CONCLUSIONS

The microsatellite markers described here will be useful for comparative study of the reproductive systems and genetic structures of the narrow endemic C. izuinsulare and the widespread C. trichotomum.

摘要

研究前提

在孤岛灌木臭牡丹（Clerodendrum izuinsulare）和与其密切相关的广泛分布的臭牡丹（C. trichotomum）中开发了核微卫星引物，为开展两种臭牡丹生殖系统和遗传结构的比较研究提供了分子工具。

方法和结果

使用改良的分离显性复合微卫星标记的方案，在臭牡丹和臭牡丹中开发了 19 对引物。引物在臭牡丹中扩增出每个位点具有一到九个等位基因的二核苷酸重复，在臭牡丹中扩增出每个位点具有一到十五个等位基因的二核苷酸重复。交叉扩增测试表明，在研究的五个其他 clerodendrum 物种中，这些标记可以扩增出四到十六个位点。