锰掺杂的 ZnSe 量子点作为荧光探针用于 5-氟尿嘧啶的时间分辨荧光检测。
Manganese-doped ZnSe quantum dots as a probe for time-resolved fluorescence detection of 5-fluorouracil.
机构信息
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China.
出版信息
Anal Chem. 2011 Dec 1;83(23):9076-81. doi: 10.1021/ac202101u. Epub 2011 Nov 9.
Quantum dots (QDs) are generally used for the conventional fluorescence detection. However, it is difficult for the QDs to be applied in time-resolved fluorometry due to their short-lived emission. In this paper, high-quality Mn-doped ZnSe QDs with long-lived emission were prepared using a green and rapid microwave-assisted synthetic approach in aqueous solution. Fluorescence lifetime of the Mn-doped ZnSe QDs was extended as long as 400 μs, which was 10,000 times higher than that of conventional QDs such as CdS, CdSe, and CdTe. The QDs exhibited an excellent photostability over 35 h under continuous irradiation at 260 nm. Capped with mercaptopropionic acid (MPA), the Mn-doped ZnSe QDs were used for the time-resolved fluorescence detection of 5-fluorouracil (5-FU) with the detection limit of 128 nM. The relative standard deviation for seven independent measurements of 1.5 μM 5-FU was 3.8%, and the recovery ranged from 93% to 106%. The results revealed that the Mn-doped ZnSe QDs could be a good candidate as a luminescence probe for highly sensitive time-resolved fluorometry.
量子点 (QDs) 通常用于常规荧光检测。然而，由于其发射寿命短，量子点很难应用于时间分辨荧光法。在本文中，我们使用绿色快速的微波辅助水相合成方法制备了具有长寿命发射的高质量 Mn 掺杂 ZnSe QDs。Mn 掺杂 ZnSe QDs 的荧光寿命延长至 400 μs 左右，是传统 QDs（如 CdS、CdSe 和 CdTe）的 10000 倍。在 260nm 连续照射下，QDs 的光稳定性超过 35 小时。巯基丙酸（MPA）封端的 Mn 掺杂 ZnSe QDs 用于 5-氟尿嘧啶（5-FU）的时间分辨荧光检测，检测限为 128 nM。对于 1.5 μM 5-FU 的七个独立测量的相对标准偏差为 3.8%，回收率范围为 93%至 106%。结果表明，Mn 掺杂 ZnSe QDs 可以作为高灵敏度时间分辨荧光法的发光探针的良好候选物。
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