Zhao Zhiyun, Liu Hui, Wang Xinli, Wang Xiaodong, Li Zhili
Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.
J Biomed Biotechnol. 2011;2011:193052. doi: 10.1155/2011/193052. Epub 2011 Oct 19.
Protein complexes are a cornerstone of many biological processes and together they form various types of molecular machinery. A broad understanding of these protein complexes is crucial for revealing and building models of protein function and regulation. Pancreatic cancer is a highly lethal disease which is difficult to diagnose at early stage and even more difficult to cure. In this study, we applied a gradient clear native gel system combined with subsequent second-dimensional SDS-PAGE to separate protein complexes from cell lysates of SW1990 and PANC-1 pancreatic cancer cell lines with different degrees of differentiation. Ten heat-shock-protein- (HSP-) associated protein complexes were separated and identified, and the differentially expressed proteins related to cancers were also found, such as HSP60, protein disulfide-isomerase A4 (ERp72), and transitional endoplasmic reticulum ATPase (TER ATPase).
蛋白质复合物是许多生物过程的基石，它们共同构成了各种类型的分子机器。对这些蛋白质复合物的广泛理解对于揭示和构建蛋白质功能及调控模型至关重要。胰腺癌是一种高度致命的疾病，早期难以诊断，更难以治愈。在本研究中，我们应用梯度清晰天然凝胶系统结合后续的二维SDS-PAGE，从不同分化程度的SW1990和PANC-1胰腺癌细胞系的细胞裂解物中分离蛋白质复合物。分离并鉴定了10种与热休克蛋白（HSP）相关的蛋白质复合物，还发现了与癌症相关的差异表达蛋白，如HSP60、蛋白质二硫键异构酶A4（ERp72）和内质网过渡ATP酶（TER ATP酶）。
