Khan M I, Rosberg S
J Cyclic Nucleotide Res. 1979;5(1):55-63.
Injection of PGF2 alpha (250 microgram/rat) 15 min prior to isolation of corpora lutea (CL) from PMSG treated immature rats significantly reduced the LH stimulation of adenylate cyclase in CL membranes. The LH stimulation did not return to normal even 24 h after PGF2 alpha injection. A transient decrease in epinephrine and fluoride stimulation of AC was also observed, the response returning to normal 6-12 h after PGF2 alpha treatment. In vitro incubation of whole isolated CL with 0.005 micrometer or higher concentrations of PGF2 alpha markedly reduced the LH and fluoride stimulation of AC in the CL membranes. Exposure of CL to PGF2 alpha for 15 min in vitro reduced the LH and fluoride response. The results are discussed in relation to the suppressive action of PGF2 alpha on LH receptors in CL, and a mechanism is proposed to explain the discrepancy in time relation between our results and the LH receptor studies. The proposed mechanism might also explain the transient decrease in epinephrine and fluoride stimulation of AC.
在从经孕马血清促性腺激素（PMSG）处理的未成熟大鼠分离黄体（CL）前15分钟注射前列腺素F2α（PGF2α，250微克/大鼠），可显著降低黄体膜中促性腺激素释放激素（LH）对腺苷酸环化酶的刺激作用。即使在注射PGF2α 24小时后，LH刺激也未恢复正常。还观察到肾上腺素和氟化物对腺苷酸环化酶（AC）刺激作用的短暂降低，在PGF2α处理后6 - 12小时反应恢复正常。用0.005微摩尔或更高浓度的PGF2α对整个分离的黄体进行体外孵育，可显著降低黄体膜中LH和氟化物对AC的刺激作用。在体外将黄体暴露于PGF2α 15分钟可降低LH和氟化物反应。结合PGF2α对黄体中LH受体的抑制作用对结果进行了讨论，并提出了一种机制来解释我们的结果与LH受体研究在时间关系上的差异。所提出的机制也可能解释肾上腺素和氟化物对AC刺激作用的短暂降低。
