超快双脉冲激发的选择性双光子荧光抑制：通过选择性单波长受激辐射进行控制。
Selective two-photon fluorescence suppression by ultrafast pulse-pair excitation: control by selective one-color stimulated emission.
机构信息
Indian Institute of Technology Kanpur, Department of Chemistry, UP-208016, India.
出版信息
J Biomed Opt. 2011 Oct;16(10):100505. doi: 10.1117/1.3645082.
Abstract
Controlling two-photon molecular fluorescence leading to selective fluorophore excitation has been a long sought after goal in fluorescence microscopy. In this letter, we thoroughly explore selective fluorescence suppression through simultaneous two-photon absorption by two different fluorophores followed by selective one-photon stimulated emission for one particular fluorophore. We achieve this by precisely controlling the time delay between two identical ultrafast near infrared laser pulses.
摘要
在荧光显微镜中，控制双光子分子荧光以实现选择性荧光团激发一直是人们长期追求的目标。在这封信中，我们通过同时双光子吸收两个不同的荧光团，然后选择性地对一个特定的荧光团进行单光子受激发射，彻底探索了通过同时双光子吸收来选择性抑制荧光的方法。我们通过精确控制两个相同的超快近红外激光脉冲之间的时间延迟来实现这一点。
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