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本文引用的文献

1
Selective suppression of two-photon fluorescence in laser scanning microscopy by ultrafast pulse-train excitation.超快脉冲串激发选择性抑制激光扫描显微镜中的双光子荧光。
J Biomed Opt. 2010 Nov-Dec;15(6):060502. doi: 10.1117/1.3509383.
2
How shaped light discriminates nearly identical biochromophores.形状如何区分几乎相同的生色团。
Phys Rev Lett. 2010 Aug 13;105(7):073003. doi: 10.1103/PhysRevLett.105.073003. Epub 2010 Aug 12.
3
Ultrafast pulse-pair control in multiphoton fluorescence laser-scanning microscopy.多光子荧光激光扫描显微镜中的超快脉冲对控制。
J Biomed Opt. 2009 Nov-Dec;14(6):064018. doi: 10.1117/1.3268440.
4
Imaging chromophores with undetectable fluorescence by stimulated emission microscopy.通过受激发射显微镜对荧光不可检测的发色团进行成像。
Nature. 2009 Oct 22;461(7267):1105-9. doi: 10.1038/nature08438.
5
Two-photon excitation STED microscopy.双光子激发受激发射损耗显微镜术
Opt Express. 2009 Aug 17;17(17):14567-73. doi: 10.1364/oe.17.014567.
6
Quantum control of tightly competitive product channels.紧密竞争产物通道的量子控制
Phys Rev Lett. 2009 Jun 26;102(25):253001. doi: 10.1103/PhysRevLett.102.253001. Epub 2009 Jun 22.
7
Multifarious control of two-photon excitation of multiple fluorophores achieved by phase modulation of ultra-broadband laser pulses.通过超宽带激光脉冲的相位调制实现对多种荧光团双光子激发的多种控制。
Opt Express. 2009 Aug 3;17(16):13737-46. doi: 10.1364/oe.17.013737.
8
Use of coherent control for selective two-photon fluorescence microscopy in live organisms.相干控制在活生物体选择性双光子荧光显微镜中的应用。
Opt Express. 2006 Jan 23;14(2):759-66. doi: 10.1364/opex.14.000759.
9
Selective two-photon microscopy with shaped femtosecond pulses.采用整形飞秒脉冲的选择性双光子显微镜术。
Opt Express. 2003 Jul 14;11(14):1695-701. doi: 10.1364/oe.11.001695.
10
Use of coherent control methods through scattering biological tissue to achieve functional imaging.通过散射生物组织使用相干控制方法来实现功能成像。
Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):16996-7001. doi: 10.1073/pnas.0407733101. Epub 2004 Nov 29.

超快双脉冲激发的选择性双光子荧光抑制：通过选择性单波长受激辐射进行控制。

Selective two-photon fluorescence suppression by ultrafast pulse-pair excitation: control by selective one-color stimulated emission.

机构信息

Indian Institute of Technology Kanpur, Department of Chemistry, UP-208016, India.

出版信息

J Biomed Opt. 2011 Oct;16(10):100505. doi: 10.1117/1.3645082.

DOI:10.1117/1.3645082
PMID:22029344
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3684794/
Abstract

Controlling two-photon molecular fluorescence leading to selective fluorophore excitation has been a long sought after goal in fluorescence microscopy. In this letter, we thoroughly explore selective fluorescence suppression through simultaneous two-photon absorption by two different fluorophores followed by selective one-photon stimulated emission for one particular fluorophore. We achieve this by precisely controlling the time delay between two identical ultrafast near infrared laser pulses.

摘要

在荧光显微镜中，控制双光子分子荧光以实现选择性荧光团激发一直是人们长期追求的目标。在这封信中，我们通过同时双光子吸收两个不同的荧光团，然后选择性地对一个特定的荧光团进行单光子受激发射，彻底探索了通过同时双光子吸收来选择性抑制荧光的方法。我们通过精确控制两个相同的超快近红外激光脉冲之间的时间延迟来实现这一点。