School of Life Sciences, Tsinghua University, Beijing, China.
FEMS Microbiol Lett. 2012 Jan;326(1):31-9. doi: 10.1111/j.1574-6968.2011.02426.x.
Per-ARNT-Sim (PAS) domains are important signalling modules that possibly monitor changes in various stimuli such as light. For the majority of PAS domains that have been identified by sequence similarity, the biological function of the signalling pathways has not yet been experimentally investigated.Thirty-three PAS proteins were discovered in Xanthomonas campestris pv. campestris(Xcc) by genome/proteome analysis. Thirteen PAS proteins were identified as contributing to light signalling and Xcc growth, motility or virulence using molecular genetics and bioinformatics methods. The PAS domains played important roles in light signalling to regulate the growth, motility and virulence of Xcc. They might be regulated by not only light quality (wavelength)but also quantity (intensity) as potential light-signalling components. Evaluating the light wavelength, three light-signalling types of PAS proteins in Xcc were shown to be involved in blue light signalling, tricolour (blue, red and far red)signalling or red/far-red signalling. This showed that Xcc had evolved a complicated light-signalling system to adapt to a complex environment.
过氧物酶-芳香烃受体核转位蛋白（PAS）结构域是重要的信号模块，可能监测各种刺激物（如光）的变化。对于通过序列相似性鉴定的大多数 PAS 结构域，其信号通路的生物学功能尚未通过实验进行研究。通过基因组/蛋白质组分析，在野油菜黄单胞菌 pv. 野油菜（Xcc）中发现了 33 种 PAS 蛋白。使用分子遗传学和生物信息学方法，确定了 13 种 PAS 蛋白参与光信号转导，并影响 Xcc 的生长、运动性或毒性。PAS 结构域在光信号转导中发挥了重要作用，调节了 Xcc 的生长、运动性和毒性。它们可能不仅受到光质（波长）的调节，还受到光量（强度）的调节，作为潜在的光信号成分。评估光波长后，Xcc 中的三种 PAS 蛋白的光信号类型被证明参与蓝光信号转导、三色（蓝、红和远红）信号转导或红/远红信号转导。这表明 Xcc 已经进化出了复杂的光信号系统，以适应复杂的环境。
