Wang Zhaopeng, Zhang Weidong, Wu Licun, Jia Qing, Wang Zhaoxia, Zhang Yueying, Ning Yunna
Department of Pathology, Institute of Basic Medicine, Shandong Academy of Medical Science, Key Laboratory for Modern Medicine and Technology of Shandong Province, Jinan 250062, China.
Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(13):1803-7.
To study the expression of HIF-1alpha and SDF-1/CXCR4 in repopulating H22 tumor tissue and the mechanism of angiogenesis of polypeptide extract from scorpion venom (PESV) during chemotherapy treatment.
The expression of HIF-1alpha and SDF-1/CXCR4 in H22 tumor tissue was monitored by immunohistochemistry, and the expression level was determined by Qwin V3 image analyzing software. The correlation between HIF-1alpha and SDF-1 was analyzed. SDF-1 content was detected by ELISA.
HIF-1alpha expression was found no difference in model group between 14 d and 21 d, and up-regulated in 28 d. There was no change of HIF-1alpha expression was observed in low-dose PESV group. In high-dose PESV group, the level of HIF-1alpha expression was high in 14 d and low in 21 d. ELISA detecting showed SDF-1 content increased slowly from 14 d to 21 d, highly from 21 d to 28 d. But in high-dose PESV groups, the content increased slowly all the time. The immunohitochemistry method got the same result with ELISA. Correlation analysis showed r = 0.805. CXCR4 expression down-regulated in two PESV treated groups, and no difference was found between these two groups.
HIF-1alpha and SDF-1 participated in VEGF expression and angiogenesis in tumor tissue during chemotherapy, while PESV could inhibit the expression of HIF-1alpha and SDF-I.
研究化疗过程中蝎毒多肽提取物（PESV）对H22肿瘤组织中缺氧诱导因子-1α（HIF-1α）、基质细胞衍生因子-1（SDF-1）/CXC趋化因子受体4（CXCR4）表达及血管生成机制的影响。
采用免疫组化法检测H22肿瘤组织中HIF-1α和SDF-1/CXCR4的表达，并用Qwin V3图像分析软件测定其表达水平。分析HIF-1α与SDF-1的相关性。采用酶联免疫吸附测定（ELISA）法检测SDF-1含量。
模型组HIF-1α在14 d和21 d表达无差异，28 d上调。低剂量PESV组HIF-1α表达无变化。高剂量PESV组HIF-1α在14 d表达高，21 d表达低。ELISA检测显示，SDF-1含量在14 d至21 d缓慢升高，21 d至28 d快速升高。但高剂量PESV组SDF-1含量一直缓慢升高。免疫组化法与ELISA法结果一致。相关性分析显示r = 0.805。两个PESV治疗组CXCR4表达下调，两组间无差异。
化疗过程中HIF-1α和SDF-1参与肿瘤组织中血管内皮生长因子（VEGF）表达及血管生成，而PESV可抑制HIF-1α和SDF-1表达。
