Institute of Pathology, University Hospital Basel, Basel, Switzerland.
Histopathology. 2011 Sep;59(3):390-6. doi: 10.1111/j.1365-2559.2011.03962.x.
Simple bone cysts (SBC) are benign tumour-like lesions, generally occurring in the metaphyses of long bones before skeletal maturity. Remarkably, in 10-70% of cases, a peculiar, amorphous and hypocellular matrix is found in the walls of SBCs which is usually regarded to consist of (calcified) fibrin clots in the literature. Because these deposits are strongly fuchsinophilic in routine van Gieson stains, the aim of this study was to investigate a series of SBCs using immunohistochemistry and electron microscopy.
A comprehensive panel of antibodies against fibrin as well as collageneous and non-collageneous proteins of bone was used, and detected substantial amounts of collagen and decorin as the main components of the investigated matrix. Electron microscopy clearly underlined the immunohistochemical results and also showed abundant fibrils with a periodic banding characteristic of collagen. Adjacent to and in between these collagen deposits runx-2- and osterix-expressing cells were detectable, most probably representing immature osteoprogenitor cells.
Although still stated in the literature and most current textbooks, we were not able to detect any evidence of fibrin as a component of the respective matrix deposits that seem to consist predominantly of collagen and decorin.
单纯性骨囊肿（SBC）是一种良性肿瘤样病变，通常发生在长骨骨干成熟之前。值得注意的是，在 10-70%的病例中，SBC 壁中存在一种特殊的、无定形和低细胞基质，文献中通常认为该基质由（钙化）纤维蛋白血栓组成。由于这些沉积物在常规的范吉森染色中呈强烈的富马精染，因此本研究的目的是使用免疫组织化学和电子显微镜检查一系列 SBC。
使用了针对纤维蛋白以及骨胶原和非胶原蛋白的综合抗体面板，并检测到大量的胶原蛋白和饰胶蛋白作为所研究基质的主要成分。电子显微镜清楚地强调了免疫组织化学结果，并显示了大量具有胶原特征周期性带的纤维。在这些胶原沉积物的相邻和之间，可以检测到表达 runt 相关转录因子 2（runx-2）和骨形成蛋白 2（osterix）的细胞，这些细胞很可能代表不成熟的成骨前体细胞。
尽管在文献和大多数当前的教科书中仍有描述，但我们无法检测到任何纤维蛋白作为相应基质沉积物成分的证据，这些基质沉积物似乎主要由胶原蛋白和饰胶蛋白组成。
