Tvrda E, Massanyi P, Lukac N, Danko J, Chrenek P
Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovakia.
Anat Histol Embryol. 2012 Jun;41(3):233-6. doi: 10.1111/j.1439-0264.2011.01116.x. Epub 2011 Oct 31.
This study was aimed to compare structures of the thyroid tissue of transgenic rabbits expressing the human clotting factor VIII under the murine whey acidic protein promoter (mWAP-hFVIII rabbits) with the non-transgenic controls. Thyroid tissue samples were taken from transgenic and non-transgenic New Zealand White rabbits, examined by optical microscopy and analysed morphometrically. The analysis revealed no significant differences (P > 0.05) in the relative volume of basic thyroid structures. Furthermore, no significant differences (P > 0.05) were observed when measuring the epithelial height and nuclear diameter of the follicular cells. Altogether, this study demonstrates no negative effect of the mWAP-hFVIII transgenesis on the rabbit thyroid gland structure.
本研究旨在比较在鼠乳清酸性蛋白启动子（mWAP-hFVIII兔）控制下表达人凝血因子VIII的转基因兔与非转基因对照兔的甲状腺组织结构。从转基因和非转基因新西兰白兔身上采集甲状腺组织样本，通过光学显微镜检查并进行形态计量分析。分析结果显示，甲状腺基本结构的相对体积无显著差异（P>0.05）。此外，在测量滤泡细胞的上皮高度和核直径时，也未观察到显著差异（P>0.05）。总之，本研究表明mWAP-hFVIII转基因对兔甲状腺结构没有负面影响。
