[Possibilities of differential diagnosis of B-cell chronic lymphoproliferative diseases using monoclonal antibodies].

Immunological phenotyping of lymphoid cells of the peripheral blood and, in some cases, of the lymph nodes and spleen was conducted in 41 patients with B-cell chronic lymphoproliferative diseases. Four monoclonal antibodies (MCA) were used in the immunofluorescence test, the results of which were analyzed by means of microscopy and flow cytofluorometry as well as by rosette-formation with mouse red blood cells. Varying expression degree has been shown by two B-cell differentiation antigens identifiable with MCA CD24 and CD20 on lymphoid cells of patients with typical B-cell chronic lymphoid leukemia (B-CLL) and lymphosarcoma. The varying expression degree is characteristic of small lymphocytes and transformed lymphoid cells in both the diseases. Differences in the number of cells expressing CD 5 (T1) antigen in B-CLL and lymphosarcoma, as well as correlation of this marker with EM-receptor expression have been recorded. The possibilities of using the results of the study for the differential diagnosis of chronic B-cell lymphoproliferative diseases, including B-CLL and B-lymphocytic lymphoma, and for investigation of B-cell lymphoproliferative disease pathogenesis have been discussed.