Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing Engineering Lab. in Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, China.
Burns. 2012 May;38(3):414-20. doi: 10.1016/j.burns.2011.09.002. Epub 2011 Oct 28.
The aim of this study was to investigate the role of substrate stiffness on the proliferation, migration, and differentiation of epidermal cells. To investigate the effects of substrate stiffness on wound healing, epidermal cells were chosen and inoculated on silicone substrate with different values of Young's modulus of elasticity. The cell growth curve, MTT method, and cell cycle detection were used to investigate proliferation, and the scratch test was used to investigate cell migration. Fluorescence flow cytometry was used to study epidermal cell differentiation. The proliferation and migration of epidermal cells favoured stiffer surfaces. A highly stiff surface stimulated epidermal cell proliferation and migration and increased re-epithelialisation, but inhibited differentiation. The candidate pathways mediating epidermal cell proliferation and migration are linked to cell anchoring to substrates by integrin-mediated focal adhesion.
本研究旨在探讨基底硬度对表皮细胞增殖、迁移和分化的作用。为了研究基底硬度对伤口愈合的影响，选择表皮细胞并接种在不同弹性模量的硅酮基底上。采用细胞生长曲线、MTT 法和细胞周期检测法研究增殖，划痕实验研究细胞迁移。荧光流式细胞术用于研究表皮细胞分化。表皮细胞的增殖和迁移有利于更硬的表面。高硬度表面刺激表皮细胞增殖和迁移，增加再上皮化，但抑制分化。介导表皮细胞增殖和迁移的候选途径与整合素介导的局灶黏附将细胞锚定在基底上有关。
