Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai, PR China.
Oncol Rep. 2012 Feb;27(2):523-8. doi: 10.3892/or.2011.1524. Epub 2011 Oct 27.
As a phytochemical derived from the roots of Salvia miltiorrhiza Bunge, Tanshinone IIA has been reported to possess anti-inflammatory and antioxidant activity. Studies in breast, colon, prostate and lung cancer indicate that Tanshinone IIA may exhibit a promising antitumor activity. However, systemic studies of the cytotoxic effects of Tanshinone IIA on gastric cancer have not been described. The present study offers a comprehensive evaluation of the antitumor effects of Tanshinone IIA in gastric cancer cells in vitro and in a mouse xenograft model. Cell viability and apoptosis in vitro were evaluated through the MTT assay and flow cytometry analysis. The results indicate that Tanshinone IIA can induce gastric cancer cell growth inhibition and apoptosis in a time- and concentration-dependent manner. Furthermore, we investigated the mechanism of the apoptotic effects induced by Tanshinone IIA. We found that Tanshinone IIA can not only cause cell cycle arrest in the G2/M phase, but also trigger the intrinsic apoptotic signaling pathway. The results suggest that Tanshinone IIA may serve as an effective adjunctive reagent in the treatment of gastric cancer.
丹参酮 IIA 是从丹参的根部提取的一种植物化学物质，据报道具有抗炎和抗氧化活性。在乳腺癌、结肠癌、前列腺癌和肺癌中的研究表明，丹参酮 IIA 可能具有有前途的抗肿瘤活性。然而，丹参酮 IIA 对胃癌的细胞毒性作用的系统研究尚未描述。本研究对丹参酮 IIA 在体外和小鼠异种移植模型中对胃癌细胞的抗肿瘤作用进行了全面评估。通过 MTT 检测和流式细胞术分析评估了细胞活力和细胞凋亡。结果表明，丹参酮 IIA 可以时间和浓度依赖的方式诱导胃癌细胞生长抑制和凋亡。此外，我们研究了丹参酮 IIA 诱导细胞凋亡的机制。我们发现，丹参酮 IIA 不仅可以引起细胞周期停滞在 G2/M 期，还可以触发内在凋亡信号通路。结果表明，丹参酮 IIA 可能是治疗胃癌的有效辅助试剂。
