Dunn W A, Siek T J
Analytic Bio-Chemistries, Inc., Feasterville, Pennsylvania 19053.
J Anal Toxicol. 1990 Jul-Aug;14(4):256. doi: 10.1093/jat/14.4.256.
A colorimetric method for the screening of cyanide in biological specimens is presented. Cyanide in a 200-microL sample is diffused in a Conway 3 compartment dish into the center well where it catalyzes the reaction between p-nitrobenzaldehyde and o-dinitrobenzene. The resulting blue-violet color intensity is proportional to cyanide concentration. The reaction is specific for cyanide, and concentrations as low as 0.05 mg/L can readily be detected.
本文介绍了一种用于生物样本中氰化物筛查的比色法。200微升样本中的氰化物在康威三室培养皿中扩散至中心孔,在那里它催化对硝基苯甲醛和邻二硝基苯之间的反应。产生的蓝紫色强度与氰化物浓度成正比。该反应对氰化物具有特异性,低至0.05毫克/升的浓度也能轻松检测到。