Vajanamarhutue C, Wilairat P, Komaratat P
J Nutr. 1979 May;109(5):848-55. doi: 10.1093/jn/109.5.848.
The effects of vitamin E deficiency on membrane integrity were studied by examining the temperature dependence of membrane-bound enzyme activities in liver mitochondria and microsome and in muscle sarcoplasmic reticulum. In vitamin E-deficient rabbits, the specific activities at 37 degrees of mitochondrial oligomycin-sensitive ATPase (EC 3.6.1.3), beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30), and microsomal glucose-6-phosphatase (EC 3.1.3.9) were increased, whereas those of microsomal NADH cytochrome C reductase (EC 1.6.99.3) and sarcoplasmic reticulum Ca-ATPase were reduced in comparison to control rabbits. Arrhenius plots of activity against temperature yielded a linear plot over the range 10 to 40 degrees in the case of beta-hydroxybutyrate dehydrogenase, NADH cytochrome C reductase and Ca-ATPase, and multiple discontinuities for glucose-6-phosphatase and oligomycin-sensitive ATPase. In control rabbits, all five enzymes showed a single discontinuity in the Arrhenius plot over the range 16 to 19 degrees. These results reflect changes in the microenvironment of membrane-bound enzymes as a consequence of vitamin E depletion.
通过检测肝线粒体、微粒体以及肌肉肌浆网中膜结合酶活性的温度依赖性，研究了维生素E缺乏对膜完整性的影响。在维生素E缺乏的兔子中，37℃时线粒体寡霉素敏感ATP酶（EC 3.6.1.3）、β-羟基丁酸脱氢酶（EC 1.1.1.30）以及微粒体葡萄糖-6-磷酸酶（EC 3.1.3.9）的比活性增加，而与对照兔子相比，微粒体NADH细胞色素C还原酶（EC 1.6.99.3）和肌浆网Ca-ATP酶的比活性降低。对于β-羟基丁酸脱氢酶、NADH细胞色素C还原酶和Ca-ATP酶，活性对温度的阿累尼乌斯曲线在10至40℃范围内呈线性，而葡萄糖-6-磷酸酶和寡霉素敏感ATP酶则有多个不连续点。在对照兔子中，所有这五种酶在16至19℃范围内的阿累尼乌斯曲线中均显示出单一的不连续点。这些结果反映了由于维生素E耗竭导致的膜结合酶微环境的变化。
