Leprince D, Gesquiere J C, Stehelin D
INSERM U186/CNRS URA 1160, Institut Pasteur, Lille, France.
Oncogene Res. 1990;5(4):255-65.
The chicken cellular c-ets-1 locus encodes for two related proteins generated by alternative splicing: the widely expressed p54c-ets-1 protein and the cellular homolog of the v-ets-encoded domain of the E26-transforming protein P135gag-myb-ets, p68c-ets-1 which has been found so far only in the spleen. We have prepared a new site specific antiserum directed against the amino-terminus of p68c-ets-1, which is highly hydrophobic by contrast to the hydrophilic NH2 terminus of p54c-ets-1. This antiserum specifically immunoprecipitated p68c-ets-1 and P135gag-myb-ets only in denaturing and reducing conditions. Despite these biochemical differences at their amino-terminal parts, p68c-ets-1, as p54c-ets-1, is a nuclear protein able to bind to DNA in vitro. Unlike p54c-ets-1 which is expressed at high levels in T- and B-lymphoid cells, p68c-ets-1 is not expressed in lymphoid cells of the spleen. Thus, the two c-ets-1 encoded proteins, although both exhibiting DNA-binding properties in vitro, display differences both in the nature of their specific NH2 termini, and in their level and pattern of expression.
鸡细胞c-ets-1基因座通过可变剪接编码两种相关蛋白：广泛表达的p54c-ets-1蛋白和E26转化蛋白P135gag-myb-ets的v-ets编码结构域的细胞同源物p68c-ets-1，到目前为止仅在脾脏中发现。我们制备了一种针对p68c-ets-1氨基末端的新的位点特异性抗血清，与p54c-ets-1的亲水性NH2末端相比，p68c-ets-1的氨基末端具有高度疏水性。这种抗血清仅在变性和还原条件下特异性免疫沉淀p68c-ets-1和P135gag-myb-ets。尽管它们在氨基末端部分存在这些生化差异，但p68c-ets-1与p54c-ets-1一样，是一种能够在体外与DNA结合的核蛋白。与在T和B淋巴细胞中高水平表达的p54c-ets-1不同，p68c-ets-1在脾脏的淋巴细胞中不表达。因此，两种c-ets-1编码的蛋白虽然在体外均表现出DNA结合特性，但在其特定NH2末端的性质、表达水平和模式上都存在差异。
