Wu Bing, Wei Yan, Liu Feng-Qiang, Zhang Qian, Wang Cun-Bang, Bai Hai
Institute of Hematology, Lanzhou Military Area General Hospital, Lanzhou, Gansu Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Oct;19(5):1214-7.
Recent studies have shown that low dose X-irradiation shows specific effect different from high dose exposures. However, the biologic responses of bone marrow mesenchymal stem cells (BM-MSC) to low dose X-irradiation have rarely been described in the literature. This study was purposed to investigate the biologic responses of human bone marrow-derived MSC to low dose X-irradiation. The proliferation of cells was tested with MTT assay, so that the cell growth curve was drawn at 1 to 7 days. The expression of survivin mRNA was detected by RT-PCR assay; the BM-MSC DNA damage induced by X-irradiation were detected with mononuclear cell gel electrophoresis. The results indicated that the proliferative ability of BM-MSC exposed to low doses of X rays was obviously enhanced as compared with control group. The low dose X-irradiation caused the damage of DNA in X-ray dose dependent manner. X-irradiation enhanced expression of survivin in MSC. It is concluded that the low dose below 20 cGy of X-irradiation has a promoting effect on survivin expression in BM-MSC. Whether the high expression of survivin plays an important role to resist ionizing radiation needs to be further studied.
近期研究表明，低剂量X射线照射显示出与高剂量照射不同的特定效应。然而，骨髓间充质干细胞（BM-MSC）对低剂量X射线照射的生物学反应在文献中鲜有描述。本研究旨在探讨人骨髓来源的MSC对低剂量X射线照射的生物学反应。用MTT法检测细胞增殖，绘制1至7天的细胞生长曲线。用RT-PCR法检测survivin mRNA的表达；用单核细胞凝胶电泳检测X射线照射诱导的BM-MSC DNA损伤。结果表明，与对照组相比，低剂量X射线照射的BM-MSC增殖能力明显增强。低剂量X射线照射以X射线剂量依赖的方式导致DNA损伤。X射线照射增强了MSC中survivin的表达。结论是，低于20 cGy的低剂量X射线照射对BM-MSC中survivin的表达有促进作用。survivin的高表达是否在抵抗电离辐射中起重要作用有待进一步研究。
