Department of Genetics, Universitat de València, Dr. Moliner 50, 46100 Burjassot, Spain.
J Invertebr Pathol. 2012 Jan;109(1):127-33. doi: 10.1016/j.jip.2011.10.009. Epub 2011 Oct 21.
Analysis of the transcriptome of Spodoptera exigua larvae revealed the presence of several ESTs with homology to virus of the order Picornavirales and with the highest similarity to Infectious flacherie virus (Iflaviridae) that infects Bombyx mori larvae. Iflaviridae is a recently defined family of insect-infecting viruses that consist of positive single strand RNA genomes translated into a single polyprotein of around 3000 amino acids long. Using the sequence information derived from the obtained ESTs, we have completed the genomic sequence of this virus. The novel S. exigua iflavirus (SeIV-1) has a genome of 10.3 kb and codes for a 3222 aa polyprotein. Expression analysis has revealed the presence of the virus in all tissues tested and insect stages, being more abundant in the midgut of the larvae. High infectivity of this virus against S. exigua has been demonstrated after observing the presence of this virus in different colonies that were reared in the same chamber with the virus-infected colony, despite no evidence of pathological effects. Further study of viral covert infections of SeIV-1 could lead to a better understanding of its pathological effect as well as any possible interaction with other microbial pathogens used for the control of this pest.
对甜菜夜蛾幼虫转录组的分析显示，存在几种与小 RNA 病毒目病毒具有同源性的 EST，与感染家蚕幼虫的传染性萎蔫病毒（杆状病毒科）的相似度最高。杆状病毒科是最近定义的昆虫感染病毒科，由正单链 RNA 基因组组成，翻译成大约 3000 个氨基酸长的单个多蛋白。利用从获得的 EST 中获得的序列信息，我们完成了该病毒的基因组序列。新型甜菜夜蛾 iflavirus（SeIV-1）的基因组为 10.3 kb，编码 3222 个氨基酸的多蛋白。表达分析表明，该病毒存在于所有测试的组织和昆虫阶段，在幼虫的中肠中更为丰富。在同一室内用感染病毒的幼虫饲养不同的品系时，观察到该病毒存在于不同的品系中，证明了该病毒对甜菜夜蛾具有高感染力，尽管没有病理效应的证据。对 SeIV-1 病毒隐性感染的进一步研究，可以更好地了解其病理效应以及与用于控制这种害虫的其他微生物病原体的任何可能相互作用。
