Thompson S M, Middleton M, Farook M, Cameron-Smith A, Bone S, Hassan A
St Peter's Hospital, Chertsey, UK.
Ann R Coll Surg Engl. 2011 Nov;93(8):589-90. doi: 10.1308/147870811X13137608455334.
Surgical tourniquets are commonplace in lower limb surgery. Several studies have shown that tourniquets can be a potential source of microbial contamination but have not compared the use of sterile versus non-sterile tourniquets in the same procedures.
Patients undergoing elective orthopaedic lower limb surgery were randomised prospectively to use of non-sterile pneumatic tourniquet or sterile elastic exsanguination tourniquet (S-MART™, OHK Medical Devices, haifa, Israel). Samples were taken from the ties of the non-sterile tourniquet prior to surgery and from the sterile tourniquets at the end of the operation in a sterile fashion. These were then sealed in universal containers and immediately analysed by the microbiology department on agar plates, cultured and incubated.
Thirty-four non-sterile tourniquets were sampled prior to surgical application, twenty-three of which were contaminated with several different organisms including coagulase-negative Staphylococcus spp, Staphylococcus aureus, Sphingomonas paucimobilis, Bacillus spp, and coliforms. Thirty-six sterile tourniquets were used, with no associated contamination.
There was significant contamination of 68% of orthopaedic surgical tourniquets. These are used regularly in procedures involving the placement of prosthesis and metalwork, and can act as a potential source of infection. We recommend the use of sterile single-use disposable tourniquets where possible. The availability of an alternative should now set the new standard of care and we recommend adopting this as a current NICE guideline for control of surgical site infection.
手术止血带在下肢手术中很常见。多项研究表明,止血带可能是微生物污染的潜在来源,但尚未在相同手术中比较使用无菌止血带与非无菌止血带的情况。
接受择期骨科下肢手术的患者被前瞻性随机分组,分别使用非无菌气动止血带或无菌弹性驱血止血带(S-MART™,OHK医疗器械公司,海法,以色列)。在手术前从非无菌止血带的绑带处取样,在手术结束时以无菌方式从无菌止血带取样。然后将这些样本密封在通用容器中,立即由微生物学部门在琼脂平板上进行分析、培养和孵育。
在手术应用前对34条非无菌止血带进行了取样,其中23条被多种不同微生物污染,包括凝固酶阴性葡萄球菌属、金黄色葡萄球菌、少动鞘氨醇单胞菌、芽孢杆菌属和大肠菌群。使用了36条无菌止血带,未发现相关污染。
68%的骨科手术止血带存在严重污染。这些止血带经常用于涉及假体和金属植入的手术中,可能成为感染的潜在来源。我们建议尽可能使用无菌一次性止血带。现在有了替代方案,应设定新的护理标准,我们建议将此作为当前英国国家卫生与临床优化研究所(NICE)控制手术部位感染的指南。