Zhang Xi, Zhu Changxiong, Zhu Honghui
Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, China.
Wei Sheng Wu Xue Bao. 2011 Jul;51(7):863-8.
Bacteroidales has been proposed as a fecal pollution indicator. microbial source tracking (MST) based on Bacteroidales host-specific gene markers has recently been applied in the fecal pollution identification, which does not require culturing the fecal pollution indicator organisms. This method needs to design specific primers. The primers are designed based on Bacteroidales specific 16S rRNA gene. Once a pair of specific primers was amplified, the fecal pollution can be identified. In this paper, the progress of specific primers of Bacteroidales in human, swine, ruminant feces were reviewed and discussed. The advantages and disadvantages were put forward. Future researchers should be focused on the new biological markers and the combination of different MST methods.
拟杆菌目已被提议作为粪便污染指标。基于拟杆菌目宿主特异性基因标记的微生物源追踪（MST）最近已应用于粪便污染识别，该方法不需要培养粪便污染指示生物。此方法需要设计特异性引物。这些引物基于拟杆菌目的特异性16S rRNA基因进行设计。一旦扩增出一对特异性引物，就可以识别粪便污染。本文综述并讨论了拟杆菌目在人、猪、反刍动物粪便中特异性引物的研究进展。提出了其优缺点。未来的研究应聚焦于新的生物标志物以及不同MST方法的联合应用。
