Bender J G, Stewart C C, Van Epps D E, Walker D E
Applied Sciences, Baxter Healthcare Corporation, Round Lake, Illinois 60073.
Exp Hematol. 1990 Oct;18(9):990-4.
Mouse bone marrow cells in liquid culture with interleukin 3 generate nonadherent granulocytes, mast cells, and macrophages. The addition of 13-cis retinoic acid (13cRA) (10(-8)-10(-6) M) enhanced proliferation of the nonadherent cells, and concentrations greater than 5 x 10(-7) M stimulated a sixfold increase in adherent macrophages. Four-color flow cytometry was used to identify the lineages present using the following antibodies: MAC1 (granulocytes and macrophages), F4/80 (macrophages), B54.2 (mast cells), and H12 (anti-Thy1.2 to identify myeloid precursors). This analysis demonstrated a twofold increase in MAC1+ F4/80+ cells, which were sorted and identified morphologically as macrophages. 13cRA also increased by 60%-95% the numbers of colony-forming cells responsive to interleukin 3 (IL-3) and macrophage colony-stimulating factor (M-CSF) but did not significantly change the colony-forming cells responsive to granulocyte-macrophage colony-stimulating factor (GM-CSF). These data suggest that 13cRA increases the production of macrophages by modulating the commitment of IL-3-expanded progenitor cells to the macrophage lineage.
在含有白细胞介素3的液体培养基中培养的小鼠骨髓细胞可生成非贴壁粒细胞、肥大细胞和巨噬细胞。添加13 - 顺式维甲酸（13cRA）（10⁻⁸ - 10⁻⁶ M）可增强非贴壁细胞的增殖，浓度大于5×10⁻⁷ M时可刺激贴壁巨噬细胞增加六倍。使用四色流式细胞术，通过以下抗体鉴定存在的细胞谱系：MAC1（粒细胞和巨噬细胞）、F4/80（巨噬细胞）、B54.2（肥大细胞）和H12（抗Thy1.2以鉴定髓系前体细胞）。该分析表明MAC1⁺F4/80⁺细胞增加了两倍，这些细胞经分选并通过形态学鉴定为巨噬细胞。13cRA还使对白介素3（IL - 3）和巨噬细胞集落刺激因子（M - CSF）有反应的集落形成细胞数量增加了60% - 95%，但对粒细胞 - 巨噬细胞集落刺激因子（GM - CSF）有反应的集落形成细胞数量没有显著改变。这些数据表明，13cRA通过调节IL - 3扩增的祖细胞向巨噬细胞谱系的分化来增加巨噬细胞的产生。
