Wolff Tanya
Cold Spring Harb Protoc. 2011 Nov 1;2011(11):1383-5. doi: 10.1101/pdb.prot066506.
Scanning electron microscopy (SEM) allows for high-magnification analysis of the external structures of the Drosophila eye. In this article, three methods are provided: one for eyes that are to be critical-point-dried (CPD) and two alternatives if a critical point drier is not available. The major difference between the first method and the other two is a preliminary fixation step. Drosophila eyes have a tendency to collapse when CPD, so fixing the animals before critical point drying improves the yield of good specimens.
扫描电子显微镜（SEM）可用于对果蝇眼睛的外部结构进行高倍分析。本文提供了三种方法：一种用于经过临界点干燥（CPD）的眼睛，另外两种是在没有临界点干燥器的情况下的替代方法。第一种方法与其他两种方法的主要区别在于一个初步固定步骤。果蝇眼睛在进行临界点干燥时容易塌陷，因此在临界点干燥之前对动物进行固定可提高获得优质标本的成功率。
