Institute of Physiological Chemistry, University Hospital Essen, Hufelandstrasse 55, Essen, Germany.
J Microencapsul. 2012;29(1):30-8. doi: 10.3109/02652048.2011.629743. Epub 2011 Nov 2.
Recently, we demonstrated that biodegradable poly(lactide-co-glycolide) (PLGA) micro- and nanocapsules with a liquid content of perfluorodecalin are principally useful for the development of artificial oxygen carriers. In order to solve a decisive and well-known problem with PLGA microcapsules, i.e. the spontaneous agglomeration of the capsules after depletion of the emulsifying agent (i.e. cholate), coating with the ABA block copolymer, Tetronic-908 was studied. After Tetronic-908 treatment at concentrations that were harmless to cultured cells, the clustering of the microcapsules was prevented, the adsorption of opsonins was decreased and the attachment to cells was inhibited, but the oxygen transport capacity of PLGA microcapsules was even increased. The present data clearly show that perfluorodecalin-filled PLGA microcapsules must be coated before decreasing the emulsifying agent cholate to physiological concentrations, in order to develop a solution that has the capabilities to function as a potential artificial oxygen carrier suspension.
最近，我们证明了具有全氟癸烷液体内容物的可生物降解的聚（乳酸-共-乙醇酸）（PLGA）微胶囊和纳米胶囊主要可用于开发人工氧载体。为了解决 PLGA 微胶囊的一个决定性和众所周知的问题，即乳化剂（即胆酸钠）耗尽后胶囊的自发聚集，研究了用 ABA 嵌段共聚物 Tetronic-908 进行涂层。在对培养细胞无害的浓度下用 Tetronic-908 处理后，防止了微胶囊的聚集，减少了调理素的吸附，并抑制了对细胞的附着，但 PLGA 微胶囊的氧传递能力甚至增加了。目前的数据清楚地表明，在将乳化剂胆酸钠降低至生理浓度之前，必须对全氟癸烷填充的 PLGA 微胶囊进行涂层，以开发一种能够作为潜在人工氧载体悬浮液发挥作用的溶液。
