College of Animal Science and Technology and State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China.
Bioresour Technol. 2012 Jan;103(1):173-9. doi: 10.1016/j.biortech.2011.10.013. Epub 2011 Oct 10.
The objective of this study was to examine the effects of nitrate on methane production, important fermentation characteristics, Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens, total bacteria, and methanogens using in vitro ruminal cultures. Potential adaptation of the above microbes and persistency of nitrate to mitigate CH4 production were also evaluated. Methane production was reduced by 70% at 12 μmol ml(-1) and nearly completely at ≥24 μmol ml(-1) nitrate. Production of volatile fatty acids (VFAs) was affected to different extents at different nitrate concentrations. Over a series of six consecutive cultures receiving 12 μmol ml(-1) nitrate, production of CH4 and VFA did not change significantly. R. albus and R. flavefaciens seemed to adapt to nitrate, while F. succinogenes and methanogens did not. Nitrate may be used in achieving persistent mitigation of CH4 production by ruminants.
本研究旨在通过体外瘤胃培养，研究硝酸盐对甲烷生成、重要发酵特性、琥珀酸纤维弧菌、白瘤胃球菌、黄瘤胃球菌、总细菌和产甲烷菌的影响。还评估了上述微生物的潜在适应性和硝酸盐缓解 CH4 产生的持久性。12 μmol ml(-1)硝酸盐可使甲烷生成减少 70%，≥24 μmol ml(-1)硝酸盐几乎完全抑制甲烷生成。不同浓度的硝酸盐对挥发性脂肪酸 (VFAs) 的产生有不同程度的影响。在连续 6 个接受 12 μmol ml(-1)硝酸盐的培养过程中，CH4 和 VFA 的产生没有显著变化。似乎 R. albus 和 R. flavefaciens 适应了硝酸盐，而 F. succinogenes 和产甲烷菌则没有。硝酸盐可能用于实现反刍动物甲烷产生的持续缓解。
