Vandenabeele P, Guisez Y, Declercq W, Bauw G, Vandekerckhove J, Fiers W
Laboratory of Molecular Biology, State University, Gent, Belgium.
Lymphokine Res. 1990 Fall;9(3):381-9.
We analyzed the proliferative response of the growth factor-dependent murine cell lines FDCp1, DA1-a, 32DC1, Ea3.15, 7TD1, BCL1 and of femural bone marrow cells for their sensitivity to various cytokines, viz. rhIL-1 beta, rhTNF, rhIL-2, mIL-3, rmIL-4, rmIL-5, rhIL-6, rhG-CSF and rmGM-CSF. We also tested for IL-1 and TNF-mediated cytokine secretion by several T cell lines and thymocytes. In all T cell systems, IL-1 alpha and IL-1 beta were equally active in the induction of cytokine production, except for the rat/mouse T cell hybridoma PC60. This cell line exhibited a 10-fold difference in specific activity for the induction of cytokine secretion between rhIL-1 alpha and the other human or murine IL-1 species. Furthermore, IL-1 and IL-2 synergistically induced PC60 cells to produce a factor, which was preferentially active on FDCp1-cells, provisionally called FDCp1-growth factor. SDS-PAGE analysis of partially purified FDCp1-GF showed 19 kDa and 24 kDa-associated biological activities. Amino-terminal and internal amino acid sequences of both bands were determined and on this basis, we identified FDCp1-GF as rat GM-CSF.
我们分析了依赖生长因子的小鼠细胞系FDCp1、DA1-a、32DC1、Ea3.15、7TD1、BCL1以及股骨骨髓细胞对多种细胞因子的增殖反应敏感性，这些细胞因子包括重组人白细胞介素-1β（rhIL-1β）、重组人肿瘤坏死因子（rhTNF）、重组人白细胞介素-2（rhIL-2）、小鼠白细胞介素-3（mIL-3）、重组小鼠白细胞介素-4（rmIL-4）、重组小鼠白细胞介素-5（rmIL-5）、重组人白细胞介素-6（rhIL-6）、重组人粒细胞集落刺激因子（rhG-CSF）和重组小鼠粒细胞-巨噬细胞集落刺激因子（rmGM-CSF）。我们还检测了几种T细胞系和胸腺细胞中白细胞介素-1和肿瘤坏死因子介导的细胞因子分泌情况。在所有T细胞系统中，白细胞介素-1α和白细胞介素-1β在诱导细胞因子产生方面活性相同，但大鼠/小鼠T细胞杂交瘤PC60除外。该细胞系在重组人白细胞介素-1α与其他人或小鼠白细胞介素-1种类之间，诱导细胞因子分泌的比活性存在10倍差异。此外，白细胞介素-1和白细胞介素-2协同诱导PC-60细胞产生一种因子，该因子对FDCp1细胞具有优先活性，暂称为FDCp1生长因子。对部分纯化的FDCp1-GF进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析，显示出19 kDa和24 kDa相关的生物学活性。测定了两条带的氨基末端和内部氨基酸序列，在此基础上，我们将FDCp1-GF鉴定为大鼠粒细胞-巨噬细胞集落刺激因子（GM-CSF）。
