Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan.
Biotechnol Lett. 2012 Aug;34(8):1571-7. doi: 10.1007/s10529-011-0788-0. Epub 2011 Nov 3.
Artificial extracellular matrix (ECM) proteins have been designed that have strong cell-adhesive activity and active functional units that inhibit network formation among vascular endothelial cells. A laminin-derived sequence (YIGSR) that blocks migration of vascular endothelial cells was designed to incorporate into an elastin-derived structural unit. The designed ECM fusion protein also had a cell-adhesive RGDN sequence that conferred an intense migration-inhibitory effect. The resultant ECM showed cell-adhesive activity superior to that of the synthetic YIGSR peptide and it blocked the migration of vascular endothelial cells. Furthermore, the designed ECM inhibited the angiogenic activity of a collagen gel. The engineering strategy of designing multi-functional ECM proteins could be applied to support novel tissue engineering techniques.
人工细胞外基质 (ECM) 蛋白已被设计出来，它们具有很强的细胞黏附活性和抑制血管内皮细胞网络形成的活性功能单位。设计了一种层粘连蛋白衍生序列 (YIGSR) 来阻止血管内皮细胞的迁移，并将其整合到弹性蛋白衍生的结构单元中。设计的 ECM 融合蛋白还具有细胞黏附 RGDN 序列，可赋予强烈的迁移抑制作用。所得的 ECM 显示出比合成 YIGSR 肽更强的细胞黏附活性，并能阻止血管内皮细胞的迁移。此外，设计的 ECM 抑制了胶原凝胶的血管生成活性。设计多功能 ECM 蛋白的工程策略可应用于支持新的组织工程技术。
