1996-2006 年斯里兰卡间日疟原虫氯喹转运蛋白（pfcrt）基因单倍型的分布模式。
Distribution pattern of Plasmodium falciparum chloroquine transporter (pfcrt) gene haplotypes in Sri Lanka 1996-2006.
机构信息
Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA.
出版信息
Am J Trop Med Hyg. 2011 Nov;85(5):811-4. doi: 10.4269/ajtmh.2011.11-0167.
Abstract
Widespread antimalarial resistance has been a barrier to malaria elimination efforts in Sri Lanka. Analysis of genetic markers in historic parasites may uncover trends in the spread of resistance. We examined the frequency of Plasmodium falciparum chloroquine transporter (pfcrt; codons 72-76) haplotypes in Sri Lanka in 1996-1998 and 2004-2006 using a high-resolution melting assay. Among 59 samples from 1996 to 1998, we detected the SVMNT (86%), CVMNK (10%), and CVIET (2%) haplotypes, with a positive trend in SVMNT and a negative trend in CVMNK frequency (P = 0.004) over time. Among 24 samples from 2004 to 2006, we observed only the SVMNT haplotype. This finding indicates selection for the SVMNT haplotype over time and its possible fixation in the population.
摘要
在斯里兰卡，抗疟药物广泛耐药一直是消除疟疾工作的障碍。对历史寄生虫遗传标记的分析可能揭示耐药性传播的趋势。我们使用高分辨率熔解曲线分析方法，检测了 1996-1998 年和 2004-2006 年期间斯里兰卡恶性疟原虫氯喹转运体（pfcrt；密码子 72-76）单倍型的频率。在 1996 年至 1998 年的 59 个样本中，我们检测到 SVMNT（86%）、CVMNK（10%）和 CVIET（2%）单倍型，SVMNT 的阳性趋势和 CVMNK 频率的负向趋势随时间推移（P = 0.004）。在 2004 年至 2006 年的 24 个样本中，我们只观察到 SVMNT 单倍型。这一发现表明，SVMNT 单倍型在过去的时间里被选择，并可能在人群中固定下来。
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