Microbe-based Fusion Technology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup, 580-185, Korea.
Bioprocess Biosyst Eng. 2012 Jan;35(1-2):61-7. doi: 10.1007/s00449-011-0595-y. Epub 2011 Nov 4.
Penicillium verruculosum COKE4E is a fungal strain isolated from bituminous coal. The microorganism cultivated in a minimal medium supplemented with Avicel, carboxymethylcellulose, and oat spelt xylan produced cellulase enzymes as exhibiting carboxymethylcellulase (CMCase), Avicelase, xylanase, and cellobiosidase activities. In this study, the productivity of the extracellular enzymes in the strain was evaluated by using empty palm fruit bunch fiber (EPFBF), a lignocellulosic biomass, as a substrate for solid-state bioconversion. The highest cellulase activities were observed after 6 days of fermentation at pH 6.0 and 30 °C. The enzymes were secreted as cellulosomes for the degradation of EPFBF as a sole carbon source. Focused ion beam analysis showed that P. verruculosum COKE4E produced cellulolytic enzymes that were able to effectively biodegrade EPFBF during solid-state fermentation. In this process, 6.5 U of CMCase, 6.8 U of Avicelase, and 8.8 U of xylanase per gram of dry solid EPFBF were produced. These results demonstrate that EPFBF may be a potential raw material in solid-state fermentation for the production of cellulase enzymes to be used for biofuel production.
疣孢青霉 COKE4E 是从烟煤中分离出来的一种真菌菌株。该微生物在含有微晶纤维素、羧甲基纤维素和燕麦黑麦木聚糖的最小培养基中培养，可产生纤维素酶，表现出羧甲基纤维素酶（CMCase）、微晶纤维素酶、木聚糖酶和纤维二糖酶活性。在这项研究中，使用空棕榈果串纤维（EPFBF）作为固态生物转化的底物来评估该菌株的胞外酶的生产力。在 pH 值为 6.0 和 30°C 的条件下发酵 6 天后，观察到最高的纤维素酶活性。这些酶作为纤维小体分泌出来，用于降解 EPFBF 作为唯一的碳源。聚焦离子束分析表明，疣孢青霉 COKE4E 产生的纤维素酶能够在固态发酵过程中有效地生物降解 EPFBF。在此过程中，每克干燥的 EPFBF 固体产生 6.5 U 的 CMCase、6.8 U 的微晶纤维素酶和 8.8 U 的木聚糖酶。这些结果表明，EPFBF 可能是固态发酵生产纤维素酶用于生物燃料生产的潜在原料。
