L-肌醇1-磷酸的中规模生产。
Medium scale production of L-myo-inositol 1-phosphate.
作者信息
Feth F, Wagner R, Baydoun H, Wray V, Wagner K G
机构信息
Gesellschaft für Biotechnologische Forschung, Ag. Enzymologie, Braunschweig, Federal Republic of Germany.
出版信息
Biotechnol Appl Biochem. 1990 Aug;12(4):364-9.
L-myo-Inositol-1-phosphate synthetase was purified from baker's yeast, grown in a fermenter in an inositol-deficient medium and analyzed using a new HPLC assay for inositol. This enzyme was used in a procedure, developed from methods partially described in the literature, for the medium scale production and purification of L-myo-inositol 1-phosphate. The identity and purity of the product were confirmed by 1H and 31P NMR spectroscopy.
L-肌醇-1-磷酸合成酶是从在缺乏肌醇的培养基中于发酵罐中培养的面包酵母中纯化得到的，并使用一种新的肌醇高效液相色谱分析法进行分析。该酶被用于一种从文献中部分描述的方法发展而来的程序，用于中规模生产和纯化L-肌醇1-磷酸。产物的身份和纯度通过1H和31P核磁共振光谱法得到确认。
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