School of Chemical Engineering and Advanced Materials, Newcastle University, Newcastle upon Tyne, United Kingdom.
Appl Spectrosc. 2011 Nov;65(11):1314-20. doi: 10.1366/11-06338.
In order to determine the bulk optical properties of a Bacillus subtilis culture during growth phase we investigated the effect of sample thickness on measurements taken with different measurement configurations, namely total diffuse reflectance and total diffuse transmittance. The bulk optical properties were extracted by inverting the measurements using the radiative transfer theory. While the relationship between reflectance and biomass changes with sample thickness and the intensity (absorbance) levels vary significantly for both reflectance and transmittance measurements, the extracted optical properties show consistent behavior in terms of both the relationship with biomass and magnitude. This observation indicates the potential of bulk optical properties for building models that could be more easily transferable compared to those built using raw measurements.
为了确定生长阶段枯草芽孢杆菌培养物的体光学性质，我们研究了样品厚度对不同测量配置（即总漫反射率和总漫透射率）测量结果的影响。通过辐射传输理论对测量结果进行反演来提取体光学性质。虽然反射率与生物量之间的关系随样品厚度而变化，并且反射率和透射率测量的强度（吸光度）水平差异显著，但提取的光学性质在与生物量的关系和大小方面表现出一致的行为。这一观察结果表明，与使用原始测量值建立的模型相比，体光学性质有可能建立更易于转移的模型。
