Reeves R, Spies G A, Kiefer M, Barr P J, Power M
Department of Biochemistry, Washington State University, Pullman 99164.
Gene. 1990 Jun 15;90(2):303-7. doi: 10.1016/0378-1119(90)90195-w.
A full-length (6.1-kb) human genomic pim-1 gene, together with its immediate 5'-upstream promoter sequence (Ppim) was isolated and sequenced. The human pim-1 gene shares an overall nucleotide (nt) sequence identity of 53% with the previously reported murine pim-1 gene. It consists of six exons and five introns and contains a protein-coding region that is identical in nt sequence to a full-length human pim-1 cDNA. The gene codes for a predicted Pim-1 protein of 313 amino acids (aa) with an Mr of 35,690 and a pI of 5.7. The deduced aa sequence of the human Pim-1 has 94% identity with the murine Pim-1 whereas the nt sequences of the two genes are 88% identical. All of the conserved aa residues of the mouse pim-1 gene, which are homologous to known protein kinases are conserved in the predicted human protein. The human Ppim region is very G + C-rich (69%) and shares greater than 80% identity with the murine Ppim. The Ppim has no TATA- or CAAT-box sequences but does contain a number of nt sequences similar to the putative binding sites of several presumptive transcription factors.
分离并测序了一个全长（6.1kb）的人类基因组pim-1基因及其紧邻的5'-上游启动子序列（Ppim）。人类pim-1基因与先前报道的鼠类pim-1基因的总体核苷酸（nt）序列同一性为53%。它由六个外显子和五个内含子组成，包含一个蛋白质编码区，其nt序列与全长人类pim-1 cDNA相同。该基因编码一个预测的313个氨基酸（aa）的Pim-1蛋白，Mr为35,690，pI为5.7。推导的人类Pim-1的aa序列与鼠类Pim-1有94%的同一性，而两个基因的nt序列有88%的同一性。小鼠pim-1基因中与已知蛋白激酶同源的所有保守aa残基在预测的人类蛋白中都是保守的。人类Ppim区域富含G + C（69%），与鼠类Ppim的同一性大于80%。Ppim没有TATA盒或CAAT盒序列，但确实包含一些与几种推测转录因子的假定结合位点相似的nt序列。
