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烟草花叶病毒RNA的5'端前导序列介导酵母提取物中不依赖起始因子4E，但仍依赖起始因子4A的翻译。

The 5'-leader sequence of tobacco mosaic virus RNA mediates initiation-factor-4E-independent, but still initiation-factor-4A-dependent translation in yeast extracts.

作者信息

Altmann M, Blum S, Wilson T M, Trachsel H

机构信息

Institut für Biochemie und Molekularbiologie, Universität Bern, Switzerland.

出版信息

Gene. 1990 Jul 2;91(1):127-9. doi: 10.1016/0378-1119(90)90173-o.

DOI:10.1016/0378-1119(90)90173-o
PMID:2205536
Abstract

Messenger RNAs encoding chloramphenicol acetyltransferase (CAT) with or without the 5'-leader sequence of tobacco mosaic virus (TMV) RNA were synthesized in vitro and translated in Saccharomyces cerevisiae extracts dependent on eukaryotic initiation factors eIF-4E or eIF-4A. The 5'-leader sequence of TMV RNA renders translation of CAT mRNA eIF-4E-independent but still 4A-dependent.

摘要

编码氯霉素乙酰转移酶（CAT）且带有或不带有烟草花叶病毒（TMV）RNA 5'-前导序列的信使核糖核酸（mRNA）在体外合成，并在依赖真核起始因子eIF-4E或eIF-4A的酿酒酵母提取物中进行翻译。TMV RNA的5'-前导序列使CAT mRNA的翻译不依赖eIF-4E，但仍依赖4A。

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1
The 5'-leader sequence of tobacco mosaic virus RNA mediates initiation-factor-4E-independent, but still initiation-factor-4A-dependent translation in yeast extracts.烟草花叶病毒RNA的5'端前导序列介导酵母提取物中不依赖起始因子4E，但仍依赖起始因子4A的翻译。
Gene. 1990 Jul 2;91(1):127-9. doi: 10.1016/0378-1119(90)90173-o.
2
Translation initiation factor-dependent extracts from Saccharomyces cerevisiae.来自酿酒酵母的翻译起始因子依赖性提取物。
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Evidence that the 5'-untranslated leader of mRNA affects the requirement for wheat germ initiation factors 4A, 4F, and 4G.信使核糖核酸（mRNA）5'-非翻译前导序列影响小麦胚芽起始因子4A、4F和4G需求的证据。
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Studies on the mechanism of translational enhancement by the 5'-leader sequence of tobacco mosaic virus RNA.烟草花叶病毒RNA 5'端前导序列增强翻译机制的研究。
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Characterisation of the 5'-leader sequence of tobacco mosaic virus RNA as a general enhancer of translation in vitro.烟草花叶病毒RNA 5'前导序列作为体外翻译通用增强子的特性分析。
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The ribosomal fraction mediates the translational enhancement associated with the 5'-leader of tobacco mosaic virus.核糖体部分介导了与烟草花叶病毒5'前导序列相关的翻译增强作用。
Nucleic Acids Res. 1988 Sep 12;16(17):8675-94. doi: 10.1093/nar/16.17.8675.

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