从肉类工业副产品中提取和回收蛋白质的效率。

Efficiency of protein extraction and recovery from meat industry by-products.

作者信息

Gault N F, Lawrie R A

机构信息

Food Science Laboratories, Department of Applied Biochemistry and Nutrition, University of Nottingham, Sutton Bonington, Nr. Loughborough, Leics. LE12 5RD, Great Britain.

出版信息

Meat Sci. 1980 Jul;4(3):167-90. doi: 10.1016/0309-1740(80)90047-9.

DOI:10.1016/0309-1740(80)90047-9

PMID:22055698

Abstract

Data concerning the efficiency of protein extraction from meat waste tissues are presented. The tissues investigated were the lungs, stomach and small and large intestines of the ox, sheep and pig. Practically all of the protein, with the exception of connective tissue proteins, was solubilised under optimum extraction conditions both with alkali and anionic detergent. The disadvantages of isoelectric precipitation of alkaline extracted proteins have been investigated in detail using polyacrylamide gel electrophoresis incorporating sodium dodecyl sulphate (SDS). The compositions of the protein isolates from the various tissues studied differed from those of the soluble extracts and supernatants or wheys. However, a component of MW 75,000 daltons was characteristic of the wheys from each tissue. The compositions of both isolates and wheys are discussed in the light of structural and cytoplasmic proteins present in smooth muscle tissues. The usefulness of anionic polysaccharides as a means of whey protein recovery is discussed, together with similar benefits achieved using SDS.

摘要

本文给出了从肉类废弃物组织中提取蛋白质效率的数据。所研究的组织包括牛、羊和猪的肺、胃以及小肠和大肠。实际上，除结缔组织蛋白外，几乎所有蛋白质在最佳提取条件下都能被碱和阴离子洗涤剂溶解。使用含十二烷基硫酸钠（SDS）的聚丙烯酰胺凝胶电泳详细研究了碱性提取蛋白等电沉淀的缺点。所研究的各种组织的蛋白质分离物的组成与可溶性提取物、上清液或乳清的组成不同。然而，分子量为75,000道尔顿的一种成分是每个组织乳清的特征。根据平滑肌组织中存在的结构蛋白和细胞质蛋白，对分离物和乳清的组成进行了讨论。还讨论了阴离子多糖作为回收乳清蛋白方法的实用性，以及使用SDS所取得的类似效果。