Cross H R, West R L, Dutson T R
Meat Science Research Laboratory, USDA, AR Beltsville, MD 20705, USA.
Meat Sci. 1981 Jul;5(4):261-6. doi: 10.1016/0309-1740(81)90016-4.
The objective of the study was to compare the precision of the laser method for measuring sarcomere length with the precision of two oil-immersion microscope methods. Eighteen semitendinosus muscles were assigned to one of three post-mortem treatments to provide a wide range in sarcomere length. Two 5·0cm sections were removed from the centre of each muscle. Each section was subdivided into six equal-size pieces and randomly allotted to each of three participating institutions. Analysis of variance revealed that sarcomere length measurement was not significantly affected by the method of measurement or by the technician. For 99% precision, the laser method required 34 measurements, whereas the two microscope methods required 45 and 66 measurements, respectively.
本研究的目的是比较激光测量肌节长度方法的精度与两种油浸显微镜测量方法的精度。将18块半腱肌分配到三种死后处理中的一种，以提供肌节长度的广泛范围。从每块肌肉的中心取出两个5.0厘米的切片。每个切片再细分为六个大小相等的小块，并随机分配给三个参与机构中的每一个。方差分析显示，肌节长度测量不受测量方法或技术人员的显著影响。对于99%的精度，激光测量方法需要34次测量，而两种显微镜测量方法分别需要45次和66次测量。
