米曲霉RIB 40中环匹阿尼酸生物合成基因簇的功能分析
Functional analysis of the cyclopiazonic acid biosynthesis gene cluster in Aspergillus oryzae RIB 40.
作者信息
Shinohara Yasutomo, Tokuoka Masafumi, Koyama Yasuji
机构信息
Noda Institute for Scientific Research, Noda, Chiba, Japan.
出版信息
Biosci Biotechnol Biochem. 2011;75(11):2249-52. doi: 10.1271/bbb.110467. Epub 2011 Nov 7.
The cyclopiazonic acid (CPA) nonproducing strain, Aspergillus oryzae RIB 40, does not biosynthesize cyclo-acetoacetyl-L-tryptophan (cAATrp) due to a truncation in the responsible PKS-NRPS gene. We found that RIB 40 converted cAATrp to 2-oxocyclopiazonic acid, the final product of CPA biosynthesis in A. oryzae. This indicates that the CPA biosynthesis gene cluster, except for the PKS-NRPS gene, is functional in RIB 40.
不产环匹阿尼酸（CPA）的米曲霉RIB 40菌株，由于负责的聚酮合酶-非核糖体肽合成酶（PKS-NRPS）基因发生截短，无法生物合成环乙酰乙酰-L-色氨酸（cAATrp）。我们发现，RIB 40可将cAATrp转化为2-氧代环匹阿尼酸，这是米曲霉中CPA生物合成的终产物。这表明，除PKS-NRPS基因外，CPA生物合成基因簇在RIB 40中具有功能。
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