Beaulieu Jean-François, Ménard Daniel
CIHR Team on Digestive Epithelium, Département d'anatomie et de biologie cellulaire, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, QC, Canada.
Methods Mol Biol. 2012;806:157-73. doi: 10.1007/978-1-61779-367-7_11.
The intestinal epithelium is a highly dynamic tissue undergoing constant and rapid renewal. It consists of a functional villus compartment responsible for terminal digestion and nutrient absorption and a progenitor cell compartment located in the crypts that produce new cells. The mechanisms regulating cell proliferation in the crypt, their migration, and differentiation are still incompletely understood. Until recently, normal human intestinal cell models allowing the study of these mechanisms have been lacking. In our laboratory, using fetal human intestines obtained at mid-gestation, we have generated the first normal human intestinal epithelial crypt-like (HIEC) cell line and villus-like primary cultures of differentiated enterocytes (PCDE). In this chapter, we provide a detailed description of the methodologies used to generate and characterize these normal intestinal crypt and villus cell models.
肠上皮是一种高度动态的组织，不断经历快速更新。它由负责终末消化和营养吸收的功能性绒毛区室以及位于隐窝中产生新细胞的祖细胞区室组成。调节隐窝中细胞增殖、迁移和分化的机制仍未完全了解。直到最近，仍缺乏能够研究这些机制的正常人类肠道细胞模型。在我们实验室，利用妊娠中期获得的胎儿肠道，我们建立了首个正常人类肠道上皮隐窝样（HIEC）细胞系以及分化肠上皮细胞的绒毛样原代培养物（PCDE）。在本章中，我们详细描述了用于建立和表征这些正常肠道隐窝和绒毛细胞模型的方法。
