猪血清中猪细小病毒的存在与抗体滴度无关。

Presence of porcine parvovirus in sera from pigs is independent of antibody titers.

作者信息

Streck André F, Gava Danielle, Souza Carine K, Gonçalves Karla R, Bortolozzo Fernando P, Wentz Ivo, Canal Cláudio W

机构信息

Institute for Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany.

出版信息

Berl Munch Tierarztl Wochenschr. 2011 May-Jun;124(5-6):242-6.

PMID:22059296

Abstract

Porcine parvovirus (PPV) is a widespread DNA virus that causes reproductive failure in swine. The aim of the present study was to investigate the presence of PPV in sera of nursery piglets (healthy n = 191 and wasting n = 132) and regularly vaccinated sows (with different parity rank [PR] n = 129), collected from different herds. Altogether, 452 animals were sampled in 27 herds owned by five companies. All sera were analyzed for the presence of PPV DNA by nested-PCR. The samples from sows were in addition tested for the presence of antibodies by Hemagglutination Inhibition (HI). PPV DNA was detected in healthy piglets (15.7%), wasting piglets (18.2%) and sows (17.8%). 25 herds had at least one positive sample and four companies had positive animals. The serology revealed that 84.7% of the sows had detectable antibodies and the fourth PR sows had the highest mean PPV antibody titers. Thirteen sows (19.1%) were found to be positive for DNA detection in the presence of high levels of antibody titers (> 512). This finding indicates that PPV DNA can be detected in different swine production categories irrespective of antibody titers.

摘要

猪细小病毒（PPV）是一种广泛传播的DNA病毒，可导致猪的繁殖障碍。本研究的目的是调查从不同猪群中采集的保育仔猪（健康仔猪n = 191头，消瘦仔猪n = 132头）血清以及定期接种疫苗的母猪（不同胎次 [PR] n = 129头）血清中PPV的存在情况。总共在五家公司拥有的27个猪群中对452头动物进行了采样。通过巢式PCR分析所有血清中PPV DNA的存在情况。此外，对母猪样本进行血凝抑制（HI）试验以检测抗体的存在。在健康仔猪（15.7%）、消瘦仔猪（18.2%）和母猪（17.8%）中检测到了PPV DNA。25个猪群至少有一个阳性样本，四家公司有阳性动物。血清学检测显示，84.7%的母猪具有可检测到的抗体，第四胎次的母猪平均PPV抗体效价最高。在抗体效价较高（> 512）的情况下，发现13头母猪（19.1%）DNA检测呈阳性。这一发现表明，无论抗体效价如何，均可在不同猪生产类别中检测到PPV DNA。