Chen G, Zamaratskaia G, Madej A, Lundström K
Department of Food Science, Swedish University of Agricultural Sciences, P.O. Box 7051, Uppsala SE-750 07, Sweden.
Meat Sci. 2006 Feb;72(2):339-47. doi: 10.1016/j.meatsci.2005.08.004. Epub 2005 Sep 29.
Our objective was to evaluate pigs injected with human chorionic gonadotropin (hCG) as a potential model to study the effect of high testicular steroid levels on the variation in indolic compounds. Pre-selection of the sires based on their plasma skatole levels was used, to evaluate whether the response to hCG would be affected. Out of 34 entire male pigs, 17 pigs were injected with hCG and the remaining pigs were injected with sterile saline four days prior to slaughter. HCG injection increased the levels of testicular steroids (androstenone, testosterone, dehydroepiandrosterone sulphate, oestrone sulphate and oestrone; p<0.001), and indole levels in plasma and fat (p<0.05). Skatole levels in fat increased slightly but not significantly (p=0.107), whereas skatole levels in plasma were not affected. The correlation coefficients between androstenone and skatole levels in fat were 0.54 in the hCG-injected group and non-significant in the control group. Skatole levels in fat were not correlated with testicular steroids in either hCG-injected pigs or controls. Skatole and indole levels in fat were positively correlated in both groups. We concluded that hCG-injected pigs might be useful for studying factors responsible for the role of testicular steroids in the occurrence of high indole levels. Pre-selection of sires based on plasma skatole levels affected both the levels of indolic compounds and testicular steroids, especially in fat, which might have suppressed the response to hCG. Whether hCG affects skatole or not is therefore not fully elucidated.
我们的目的是评估注射人绒毛膜促性腺激素（hCG）的猪作为一种潜在模型，以研究高睾丸类固醇水平对吲哚类化合物变化的影响。基于公猪血浆粪臭素水平进行预选，以评估对hCG的反应是否会受到影响。在34头完整雄性猪中，17头猪注射了hCG，其余猪在屠宰前四天注射了无菌生理盐水。注射hCG可提高睾丸类固醇（雄烯酮、睾酮、硫酸脱氢表雄酮、硫酸雌酮和雌酮；p<0.001）以及血浆和脂肪中吲哚水平（p<0.05）。脂肪中粪臭素水平略有升高但不显著（p=0.107），而血浆中粪臭素水平未受影响。在注射hCG的组中，雄烯酮与脂肪中粪臭素水平的相关系数为0.54，在对照组中无显著相关性。在注射hCG的猪或对照组中，脂肪中粪臭素水平与睾丸类固醇均无相关性。两组中脂肪中粪臭素和吲哚水平均呈正相关。我们得出结论，注射hCG的猪可能有助于研究睾丸类固醇在高吲哚水平发生中所起作用的相关因素。基于血浆粪臭素水平对公猪进行预选会影响吲哚类化合物和睾丸类固醇的水平，尤其是在脂肪中，这可能抑制了对hCG的反应。因此，hCG是否影响粪臭素尚未完全阐明。
