Lawrence R J, Doyle J C, Elliott R, Norton B W, Loxton I
Schools of Veterinary Science and Animal Studies, University of Queensland, Qld. 4072, Australia; Integrated Animal Production, P.O. Box 1821, Toowoomba, Qld. 4350, Australia.
Meat Sci. 2007 Oct;77(2):228-37. doi: 10.1016/j.meatsci.2007.03.013. Epub 2007 Mar 24.
Biotin (D-biotin) was supplemented to F1 Wagyu/Black Angus steers fed a wheat-based ration to evaluate the effect on meat quality. One hundred and eight steers of known Wagyu sire lines were assigned to three biotin treatments (0, 10 and 20mg/head/day) with each treatment replicated four times using an unfasted liveweight of 410.5kg (±24.42 SD). Biotin supplementation had no effect (P>0.05) on beef marbling standard at either the 5/6th or 10/11th rib quartering site, 10/11th rib intra-muscular fat percentage, intra-muscular fat fatty acid composition or adipose melting points. Wagyu genotype had an effect (P<0.05) on beef marbling standard and intra-muscular fat percentage at the 10/11th rib, inter-muscular and intra-muscular melting point and fatty acid composition of intra-muscular fat. A significant (P<0.001) but poor correlation existed between beef marbling standard and intra-muscular fat percentage (R(2)=0.198). Total conjugated linoleic acid had a highly significantly (P<0.0001) positive correlation to intra-muscular fat percentage (R(2)=0.446).
向以小麦为基础日粮饲养的F1和牛/黑安格斯阉牛补充生物素（D-生物素），以评估其对肉质的影响。108头已知和牛父系品系的阉牛被分配到三种生物素处理组（0、10和20毫克/头/天），每种处理重复四次，空腹活重为410.5千克（±24.42标准差）。在第5/6或第10/11肋切块部位的牛肉大理石花纹标准、第10/11肋肌内脂肪百分比、肌内脂肪脂肪酸组成或脂肪熔点方面，补充生物素均无影响（P>0.05）。和牛基因型对第10/11肋处的牛肉大理石花纹标准和肌内脂肪百分比、肌间和肌内熔点以及肌内脂肪的脂肪酸组成有影响（P<0.05）。牛肉大理石花纹标准与肌内脂肪百分比之间存在显著（P<0.001）但较弱的相关性（R²=0.198）。总共轭亚油酸与肌内脂肪百分比高度显著正相关（P<0.0001）（R²=0.446）。
