Department of Cardiology, Timone University Hospital, Marseille, France.
Int J Cardiol. 2013 Jun 5;166(1):225-9. doi: 10.1016/j.ijcard.2011.10.090. Epub 2011 Nov 5.
During ischaemia, the extracellular level of adenosine increases, which has cytotoxic effects. In endothelium, cell surface adenosine deaminase (ADA) complexing CD26 is coordinately induced during ischaemia as part of an adaptative response by eliminating adenosine. We examined whether a similar mechanism exists for mononuclear cells. We studied mononuclear cell surface ADA (MCADA) and dipeptidyl-peptidase IV activity (DPPIV) of membrane CD26 during percutaneous transluminal coronary angioplasty (PTCA) as a model of ischaemia-reperfusion. Enzymatic activities were compared with levels of ischaemia-modified albumin (IMA), a marker of ischaemia-reperfusion.
Patients (15 men and 5 women) with non-ST segment elevation acute coronary syndrome related to a stenosis of proximal left anterior descending artery were prospectively included before revascularization. MCADA, DPPIV and IMA were measured before PTCA (T0) then 15 (T15) and 120 (T120) minutes after reperfusion. Fifteen healthy control subjects were enrolled. At T0, MCADA and IMA levels were higher in patients than in controls. MCADA decreased at T15 (median, IQR: 8.2 [7.6-9.8] IU) relative to T0 (11.25 [10-13.5] IU, p<0.01) and remained low at T120. DPPIV decreased at T15 (0.9 [0.7-1.1] AU) relative to T0 (1.05 [0.99-1.48] AU; p<0.01) and remained low at T120. IMA level increased only at T120. MCADA and DPPIV were correlated. Our findings are that MCADA and DPPIV decreased rapidly after angioplasty, suggesting that both catalysts are early markers of reperfusion.
MCADA and DPPIV are sensitive and early markers of ischaemia-reperfusion process during PTCA.
在缺血期间，细胞外腺苷水平增加，具有细胞毒性作用。在内皮细胞中，细胞表面腺苷脱氨酶（ADA）复合物与 CD26 在缺血期间协同诱导，作为消除腺苷的适应性反应的一部分。我们研究了单核细胞是否存在类似的机制。我们研究了经皮腔内冠状动脉成形术（PTCA）作为缺血再灌注模型期间单核细胞表面 ADA（MCADA）和二肽基肽酶 IV 活性（DPPIV）作为单核细胞表面 CD26 的膜。将酶活性与缺血修饰白蛋白（IMA）的水平进行比较，IMA 是缺血再灌注的标志物。
前瞻性纳入了 15 名患有非 ST 段抬高急性冠状动脉综合征的男性和 5 名女性患者，与左前降支近端狭窄有关。在血管重建前分别于 PTCA 前（T0）、再灌注后 15 分钟（T15）和 120 分钟（T120）测量 MCADA、DPPIV 和 IMA。纳入了 15 名健康对照者。在 T0 时，患者的 MCADA 和 IMA 水平高于对照组。MCADA 在 T15 时（中位数，IQR：8.2 [7.6-9.8] IU）相对于 T0 时（11.25 [10-13.5] IU，p<0.01）下降，并在 T120 时仍较低。DPPIV 在 T15 时（0.9 [0.7-1.1] AU）相对于 T0 时（1.05 [0.99-1.48] AU，p<0.01）下降，在 T120 时仍较低。IMA 水平仅在 T120 时升高。MCADA 和 DPPIV 相关。我们的发现是，血管成形术后 MCADA 和 DPPIV 迅速下降，表明这两种催化剂都是再灌注的早期标志物。
MCADA 和 DPPIV 是 PTCA 期间缺血再灌注过程的敏感和早期标志物。
