对杜洛克猪和长白猪补充一水肌酸的体外和体内研究。

In vitro and in vivo studies of creatine monohydrate supplementation to Duroc and Landrace pigs.

作者信息

Young J F, Bertram H C, Theil P K, Petersen A-G D, Poulsen K A, Rasmussen M, Malmendal A, Nielsen N C, Vestergaard M, Oksbjerg N

机构信息

Danish Institute of Agricultural Sciences, Department of Food Science, Research Centre Foulum, Tjele, Denmark.

出版信息

Meat Sci. 2007 Jun;76(2):342-51. doi: 10.1016/j.meatsci.2006.11.015. Epub 2007 Jan 10.

DOI:10.1016/j.meatsci.2006.11.015

PMID:22064305

Abstract

Duroc and Landrace pigs as well as primary myotubes from these breeds were used to investigate mechanisms behind differences in their response to creatine monohydrate (CMH). Pigs were supplemented with 0, 12.5, 25 or 50g CMH/d for 5 days (n=10 per treatment and breed). Plasma levels of creatine increased dose-dependently in both breeds, while muscle-creatine phosphate content increased only in the Duroc pigs. (1)H NMR metabolic profiling showed a tendency towards clustering according to CMH supplementation only among Duroc pigs, revealing a stronger response compared to Landrace pigs. The abundance of insulin-like growth factor I and myostatin mRNA was decreased by CMH supplementation while that of type 1 IGF-receptor and creatine transporter was unaffected. Protein synthesis, increased in the myotubes from both breeds, indicating protein accretion, but no effect was observed on the mRNA abundance of IGF-I, type 1 IGF-receptor, myostatin or the creatine transporter in myotubes.

摘要

选用杜洛克猪和长白猪以及来自这些品种的原代肌管，来研究它们对一水肌酸（CMH）反应差异背后的机制。给猪补充0、12.5、25或50克CMH/天，持续5天（每个处理和品种n = 10）。两个品种猪的血浆肌酸水平均呈剂量依赖性增加，而肌肉磷酸肌酸含量仅在杜洛克猪中增加。氢核磁共振代谢谱分析表明，仅在杜洛克猪中，根据CMH补充情况有聚类趋势，这表明与长白猪相比，杜洛克猪的反应更强。补充CMH可降低胰岛素样生长因子I和肌肉生长抑制素mRNA的丰度，而I型胰岛素样生长因子受体和肌酸转运蛋白的丰度不受影响。两个品种的肌管中蛋白质合成均增加，表明有蛋白质积累，但未观察到CMH对肌管中IGF-I、I型IGF受体、肌肉生长抑制素或肌酸转运蛋白的mRNA丰度有影响。