Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
Ann Biomed Eng. 2012 Jun;40(6):1211-27. doi: 10.1007/s10439-011-0455-6. Epub 2011 Nov 8.
Advances in microengineering technologies have enabled a variety of insights into biomedical sciences that would not have been possible with conventional techniques. Engineering microenvironments that simulate in vivo organ systems may provide critical insight into the cellular basis for pathophysiologies, development, and homeostasis in various organs, while curtailing the high experimental costs and complexities associated with in vivo studies. In this article, we aim to survey recent attempts to extend tissue-engineered platforms toward simulating organ structure and function, and discuss the various approaches and technologies utilized in these systems. We specifically focus on microtechnologies that exploit phenomena associated with compartmentalization to create model culture systems that better represent the in vivo organ microenvironment.
微工程技术的进步使人们能够深入了解生物医学科学，如果没有传统技术，这是不可能的。模拟体内器官系统的工程微环境可能为各种器官的病理生理学、发育和内稳态的细胞基础提供重要的见解，同时减少与体内研究相关的高实验成本和复杂性。在本文中，我们旨在调查最近尝试将组织工程平台扩展到模拟器官结构和功能的方法，并讨论这些系统中使用的各种方法和技术。我们特别关注利用与分隔相关的现象来创建更能代表体内器官微环境的模型培养系统的微技术。
