Department of Marine Environment and Engineering, National Sun Yat-sen University, Kaohsiung, Taiwan.
Environ Toxicol Chem. 2012 Feb;31(2):246-52. doi: 10.1002/etc.735. Epub 2011 Dec 9.
The binding constants (K(DOC)) of the mixture of benzo[h]quinoline and its protonated analog, benzo[h]quinolinium, to four types of humic substances obtained from the International Humic Substances Society were determined by the fluorescence quenching method. A simple mixing model was used to eliminate the fluorescent interference from the minor analog in the solution and to deduce K(mix), which represents the overall binding as the sum of that for the individual analogs. The characteristics of humic substances, especially their hydrophobicity and aromaticity, established by principal component analysis of structural and elemental compositions, were the main determinants of the binding affinity with both benzo[h]quinoline and benzo[h]quinolinium (K(BQ) and K (BQH+) across a range of pH values. The strongest overall affinity of benzo[h]quinoline for humic substances is observed near pH 4 and with more hydrophobic humic substances, which suggests possible choices in attempts at remediation of benzo[h]quinoline containing particles with humic substances.
采用荧光猝灭法测定了国际腐殖质协会提供的四种腐殖质中苯并[h]喹啉及其质子化类似物苯并[h]喹啉鎓的混合物的结合常数（K(DOC)）。使用简单的混合模型消除了溶液中小分子类似物的荧光干扰，并推导出了 K(mix)，它代表了整体结合，是各类似物结合的总和。通过对结构和元素组成进行主成分分析，确定了腐殖质的特性，特别是其疏水性和芳香性，这是苯并[h]喹啉和苯并[h]喹啉鎓（K(BQ)和 K (BQH+)在不同 pH 值范围内与腐殖质结合亲和力的主要决定因素。在 pH 值接近 4 时，苯并[h]喹啉与腐殖质的整体亲和力最强，而且与疏水性更强的腐殖质的亲和力也最强，这表明在尝试用腐殖质修复含苯并[h]喹啉的颗粒时可能有一些选择。
