Bessa José, Gómez-Skarmeta José Luis
Centro Andaluz de Biología del Desarrollo (CABD), CSIC-Universidad Pablo de Olavide, Seville, Spain.
Methods Mol Biol. 2011;772:397-408. doi: 10.1007/978-1-61779-228-1_23.
Cis-regulatory sequences control when, where, and how much genes are transcribed. A better understanding on these elements is a fundamental keystone to better understand development, cell differentiation, and morphogenesis. Several methods based on in silico analysis or ChIP-seq experiments have been developed to detect cis-acting sequences. Here, we describe a protocol to isolate such sequences from genomic DNA and to clone them into expression vectors for functional assays using the Gateway cloning technology.
顺式调控序列控制基因转录的时间、位置和数量。更好地理解这些元件是更好地理解发育、细胞分化和形态发生的基本基石。已经开发了几种基于计算机分析或ChIP-seq实验的方法来检测顺式作用序列。在这里，我们描述了一种从基因组DNA中分离此类序列并将其克隆到表达载体中以使用Gateway克隆技术进行功能测定的方案。
