[大花红景天中红景天苷和多糖的制备研究]
[Study on preparation of salidroside and polysaccharide in Rhodiola crenulata].
作者信息
Wang Cong-qing, Chen Juan, Li Cai-dong, Wu Bin
机构信息
Lanzhou Institute of Liver Disease, the No. 2 People's Hospital of Lanzhou, Lanzhou 730046, China.
出版信息
Zhong Yao Cai. 2011 Jul;34(7):1122-5.
OBJECTIVE
To optimize the preparation process of salidroside and polysaccharide in Rhodiola crenulata.
METHODS
Water-extraction and alcohol-precipitation was used. The effect of added water volume, decoction time and frequency on water-extracting process was investigated by L9 (3(4)) orthogonal design using extraction rate, content of salidroside and polysaccharide as the assessment indexes. The effect of liquor strength and ethanol concentration on alcohol-precipitating process was investigated as well, using content of salidroside and polysaccharide as the assessment indexes.
RESULTS
The optimum conditions for water-extracting process were as follows: decocting 3 times, and each time adding 8 fold of water and extracting 2 h. For alcohol-precipitating process,it was that liquor strength was 0.4 g/mL, and ethanol concentration in liquor was 70%.
CONCLUSION
The optimized process has guidance for the development and comprehensive utilization of this plant.
目的
优化大花红景天中红景天苷和多糖的制备工艺。
方法
采用水提醇沉法。以提取率、红景天苷和多糖含量为评价指标，通过L9(3(4))正交试验考察加水量、煎煮时间和次数对水提取工艺的影响。同时以红景天苷和多糖含量为评价指标，考察药液浓度和乙醇浓度对醇沉工艺的影响。
结果
水提取工艺的最佳条件为：煎煮3次，每次加8倍量水，提取2小时。醇沉工艺的最佳条件为：药液浓度0.4 g/mL，药液中乙醇浓度7０%。
结论
优化后的工艺对该植物的开发和综合利用具有指导意义。
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