Department of Clinical, Toxicological and Bromatological Analysis, Ribeirão Preto School of Pharmaceutical Sciences, University of São Paulo, Av. Bandeirantes s/n, Monte Alegre, Ribeirão Preto, São Paulo, Brazil.
Climacteric. 2012 Apr;15(2):186-94. doi: 10.3109/13697137.2011.582970. Epub 2011 Nov 8.
The risks of hormone replacement therapy have led to a search for new alternatives such as phytoestrogens, plant compounds with estrogen-like biological activity. Isoflavones are the phytoestrogens most extensively studied and can be found in soybean, red clover and other plants. Due to this estrogen-like activity, phytoestrogens can have some effect on atherosclerosis. Human umbilical vein endothelial cells (HUVEC) have been extensively used to study the biology and pathobiology of human endothelial cells and most of the knowledge acquired is due to experiments with cultures of these cells.
To evaluate the effects of the phytoestrogen extracts from Glycine max soy bean, genistein, formononetin, biochanin A and daidzein, as well as a mixture of these extracts (Mix), on expression of adhesion molecules, VCAM-1, ICAM-1 and E-selectin, by endothelial cell HUVEC, stimulated with lipopolysaccharide.
HUVEC were cultured in medium EBM(2), pretreated with isoflavones for 24 and 48 h and then stimulated with lipopolysaccharide; in addition, isoflavones were added, after stimulation by lipopolysaccharide, to HUVEC. We evaluated the production of VCAM-1, ICAM-1 and E-selectin on cell surface, by cell-based enzyme immunoassay, and of sVCAM-1, sICAM-1 and sE-selectin in culture supernatant, by ELISA.
Genistein, formononetin, biochanin A and daidzein, as well as the Mix were able to reduce VCAM-1, ICAM-1 and E-selectin on cell surface and in culture supernatant. Conclusion Isoflavones extracted from Glycine max soy bean, in vitro, presented antiatherogenic effects, reducing the expression of adhesion molecules and acting as preventive agents as well as therapeutic agents.
激素替代疗法的风险促使人们寻找新的替代品，如植物雌激素，具有类似雌激素生物活性的植物化合物。异黄酮是研究最广泛的植物雌激素，可在大豆、红三叶草和其他植物中找到。由于这种类似雌激素的活性，植物雌激素可能对动脉粥样硬化有一定的影响。人脐静脉内皮细胞（HUVEC）已广泛用于研究人类内皮细胞的生物学和病理生物学，并且大部分知识是基于这些细胞的培养实验获得的。
评估从大豆 Glycine max 中提取的植物雌激素提取物、染料木黄酮、芒柄花素、大豆苷元和大豆苷，以及这些提取物的混合物（Mix）对脂多糖刺激的内皮细胞 HUVEC 表达黏附分子 VCAM-1、ICAM-1 和 E-选择素的影响。
将 HUVEC 在 EBM(2)培养基中培养，用异黄酮预处理 24 和 48 小时，然后用脂多糖刺激；此外，在脂多糖刺激后，将异黄酮添加到 HUVEC 中。我们通过细胞酶免疫测定法评估细胞表面 VCAM-1、ICAM-1 和 E-选择素的产生，以及通过 ELISA 评估培养上清液中 sVCAM-1、sICAM-1 和 sE-选择素的产生。
染料木黄酮、芒柄花素、大豆苷元和大豆苷以及混合物都能够减少细胞表面和培养上清液中的 VCAM-1、ICAM-1 和 E-选择素。结论：体外从大豆中提取的异黄酮具有抗动脉粥样硬化作用，可降低黏附分子的表达，可作为预防剂和治疗剂。
