Moon Ji-Hoi, Shin Seung-Il, Chung Jong-Hyuk, Lee Seung-Woo, Amano Atsuo, Lee Jin-Yong
Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul, Korea; 2Institute of Oral Biology, Kyung Hee University, Seoul, Korea.
FEMS Immunol Med Microbiol. 2012 Apr;64(3):425-8. doi: 10.1111/j.1574-695X.2011.00889.x. Epub 2012 Apr 1.
For more accurate PCR-based identification of Porphyromonas gingivalis harboring genotype II fimA, the most prevalent type in periodontitis patients, a new primer set was developed and evaluated. The previous type II primers hybridized to the DNA of P gingivalis strains harboring type Ib as well as type II fimA, while the new primers specifically amplified only the DNA fragment of type II fimA. In the investigation using mixed bacterial culture and 155 clinical samples from peri-implantitis patients, the new primers increased the accuracy of PCR-based detection of type II fimA by excluding false-negatives as well as false-positives.
为了更准确地基于聚合酶链反应（PCR）鉴定携带基因型II菌毛蛋白A（fimA）的牙龈卟啉单胞菌（这是牙周炎患者中最常见的类型），研发并评估了一套新的引物。先前的II型引物能与携带Ib型以及II型fimA的牙龈卟啉单胞菌菌株的DNA杂交，而新引物仅特异性扩增II型fimA的DNA片段。在使用混合细菌培养物和来自种植体周围炎患者的155份临床样本进行的研究中，新引物通过排除假阴性和假阳性结果，提高了基于PCR检测II型fimA的准确性。
