The George Washington University Medical Center, Department of Anatomy and Regenerative Biology, Washington, DC 20037, USA.
Exp Eye Res. 2011 Dec;93(6):927-36. doi: 10.1016/j.exer.2011.10.012. Epub 2011 Nov 2.
Recurrent corneal erosions are painful and put patients' vision at risk. Treatment typically begins with debridement of the area around the erosion site followed by more aggressive treatments. An in vivo mouse model has been developed that reproducibly induces recurrent epithelial erosions in wild-type mice spontaneously within two weeks after a single 1.5 mm corneal debridement wound created using a dulled-blade. This study was conducted to determine whether 1) inhibiting MMP9 function during healing after dulled-blade wounding impacts erosion development and 2) wounds made with a rotating-burr heal without erosions. Oral or topical inhibition of MMPs after dulled-blade wounding does not improve healing. Wounds made by rotating-burr heal with significantly fewer erosions than dulled-blade wounds. The localization of MMP9, β4 integrin and basement membrane proteins (LN332 and type VII collagen), immune cell influx, and reinnervation of the corneal nerves were compared after both wound types. Rotating-burr wounds remove the anterior basement membrane centrally but not at the periphery near the wound margin, induce more apoptosis of corneal stromal cells, and damage more stromal nerve fibers. Despite the fact that rotating-burr wounds do more damage to the cornea, fewer immune cells are recruited and significantly more wounds resolve completely.
复发性角膜糜烂是一种疼痛的疾病，会使患者的视力面临风险。治疗通常从对糜烂部位周围的区域进行清创开始，然后进行更积极的治疗。已经开发出一种体内小鼠模型，该模型可在单次钝刀片造成 1.5 毫米角膜清创伤口后两周内，在野生型小鼠中自发地重现复发性上皮糜烂。本研究旨在确定：1）在钝刀片创伤后愈合期间抑制 MMP9 功能是否会影响糜烂的发展；2）使用旋转钻头进行的伤口是否会无糜烂愈合。钝刀片创伤后口服或局部抑制 MMP 并不能改善愈合。与钝刀片伤口相比，使用旋转钻头进行的伤口愈合后糜烂明显减少。比较了两种伤口类型后 MMP9、β4 整合素和基底膜蛋白（LN332 和 VII 型胶原蛋白）、免疫细胞浸润和角膜神经再支配的定位。旋转钻头伤口会在中央去除前基底膜，但不会在靠近伤口边缘的周边去除，会诱导更多的角膜基质细胞凋亡，并损伤更多的基质神经纤维。尽管旋转钻头伤口对角膜的损伤更大，但招募的免疫细胞更少，并且更多的伤口完全愈合。
