Gregg N J, Bach P H
Robens Institute of Health and Safety, University of Surrey, UK.
Int J Exp Pathol. 1990 Oct;71(5):659-70.
Male MF1-nu/nu/Ola/Hsd nude mice, maintained in a gnotobiotic environment, were dosed i.p. with either 50, 100 or 200 mg/kg 2-bromoethanamine (BEA) hydrobromide to induce a model papillary necrosis. Renal histological changes were examined in semithin glycolmethacrylate resin sections at 24, 48 and 72 h after BEA treatment. The sequence of medullary changes included pyknosis of interstitial cell nuclei, increased staining of the interstitial mucopolysaccharide matrix, platelets adhering to capillary endothelium, necrosis of collecting duct epithelial cells and denudation of the covering epithelium of the papilla. This was similar to that previously described in the Wistar rat, but the time course was extended. There was also a concomitant and extensive cortical necrosis of the P2 and P3 segments of the proximal tubule, which was evident prior to the onset of renal papillary necrosis at the higher doses of BEA. Nude mice show an atypical response to BEA compared to several mouse and rat strains, the hamster and pig, that suggests unique characteristics in this athymic murine mutant.
饲养于无菌环境中的雄性MF1-nu/nu/Ola/Hsd裸鼠，经腹腔注射50、100或200mg/kg氢溴酸2-溴乙胺（BEA）以诱导乳头坏死模型。在BEA处理后24、48和72小时，对半薄甲基丙烯酸乙二醇酯树脂切片进行肾脏组织学变化检查。髓质变化的顺序包括间质细胞核固缩、间质粘多糖基质染色增加、血小板粘附于毛细血管内皮、集合管上皮细胞坏死以及乳头覆盖上皮剥脱。这与先前在Wistar大鼠中描述的情况相似，但时间进程有所延长。在较高剂量BEA时，近端小管P2和P3段还伴有广泛的皮质坏死，这在肾乳头坏死发生之前就很明显。与几种小鼠和大鼠品系、仓鼠和猪相比，裸鼠对BEA表现出非典型反应，这表明这种无胸腺小鼠突变体具有独特的特征。
