SFI Tetrapyrrole Laboratory, School of Chemistry, Trinity College Dublin, Dublin, Ireland.
J Inorg Biochem. 2011 Dec;105(12):1589-95. doi: 10.1016/j.jinorgbio.2011.08.023. Epub 2011 Sep 14.
Novel phenanthroline derivatives and their europium(III) and zinc(II) complexes have been prepared in up to 92%. In contrast to the stable zinc complexes, the europium compounds exhibit a strong luminescence in THF solution. However, quenching of the emission is observed in DMSO indicating complete dissociation of the complexes back to free ligands in this solvent. (1)H NMR studies of the Eu(III)-complexes 5 and 6 also confirmed the existence of different states depending on the solvent used. Moreover, it was found that compound 5 is stable in EtOH-PBS solutions; here a strong signal in the emission spectra corresponding to the europium ion was detected. No spectral changes were observed for the zinc(II) complexes, they were shown to be stable in the media. These metal complexes can be used as fluorescence markers for the diagnosis of oesophageal squamous carcinoma (OE21) cells at low concentrations. Cell images were acquired using the compounds 5, 7-9 as luminescent agents. The first images were taken already after 20 min incubation time at a very low concentration range (0.7-1.6 μM).
已制备出高达 92%产率的新型菲咯啉衍生物及其铕（III）和锌（II）配合物。与稳定的锌配合物相比，铕化合物在 THF 溶液中表现出强荧光。然而，在 DMSO 中观察到发射的猝灭，表明在该溶剂中配合物完全解离回游离配体。（1）H NMR 研究表明，Eu(III)-配合物 5 和 6 的存在取决于所用溶剂的不同状态。此外，发现化合物 5 在 EtOH-PBS 溶液中稳定；在发射光谱中检测到对应于铕离子的强信号。锌（II）配合物没有观察到光谱变化，它们在介质中稳定。这些金属配合物可用于低浓度下诊断食管鳞状细胞癌（OE21）细胞的荧光标记。使用化合物 5、7-9 作为发光剂获取细胞图像。在非常低的浓度范围（0.7-1.6 μM）下，仅在 20 分钟孵育时间后就获得了第一张图像。
